The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response

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	The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response
	Cheng, Shuang 1,2; Zhang, Min 1; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2010-12-01
卷号	29 期号:6 页码:972-978
关键词	Edwardsiella tarda Iron-cofactored superoxide dismutase Virulence factor Macrophage Innate immunity
英文摘要	Edwardsiella tarda is a Gram-negative bacterium that can infect a wide variety of marine and freshwater fish and cause severe economic losses worldwide. With an aim to elucidate the virulence mechanism of E. tarda, we in this study cloned and analyzed the function of an iron-cofactored superoxide dismutase (FeSOD) from a pathogenic E. tarda strain TX01 isolated from diseased fish. FeSOD is 192-residue in length and contains domain structures that are conserved among iron/manganese superoxide dismutases. Recombinant FeSOD purified from Escherichia coli exhibits apparent superoxide dismutase activity. Quantitative real-time RT-PCR analysis indicated that FeSOD expression is significantly upregulated immediately following TX01 infection of Japanese flounder (Paralichthys olivaceus) head kidney (HK) macrophages and cultured FG cells. Compared to the wild type strain TX01, the FeSOD mutant strain TXSod is (i) more sensitive to H(2)O(2)-induced oxidative damage, (ii) less resistant against serum- and macrophage-mediated bacterial killing, (iii) significantly weakened in the ability to invade into FG cells and to disseminate in fish blood and liver, and (iv) deficient in blocking macrophage respiratory burst activity and production of reactive oxygen species. Furthermore, HK macrophages infected by TXSod exhibits significantly increased expression of inflammatory cytokines compared to macrophages infected by TX01. Taken together, these results indicate that FeSOD is a virulence factor that plays an important role in the pathogenicity of E. tarda by inhibiting macrophage-mediated innate immune response. (C) 2010 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000284798400009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/32693]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Nanyang Inst Technol, Nanyang 473004, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Shuang,Zhang, Min,Sun, Li. The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(6):972-978.
APA	Cheng, Shuang,Zhang, Min,&Sun, Li.(2010).The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response.FISH & SHELLFISH IMMUNOLOGY,29(6),972-978.
MLA	Cheng, Shuang,et al."The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response".FISH & SHELLFISH IMMUNOLOGY 29.6(2010):972-978.