Conjoined Use of EM and NMR in RNA Structure Refinement

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Conjoined Use of EM and NMR in RNA Structure Refinement
	Gong, Zhou 1; Schwieters, Charles D.2; Tang, Chun 1
刊名	PLOS ONE
	2015-03-23
卷号	10 期号:3
英文摘要	More than 40% of the RNA structures have been determined using nuclear magnetic resonance (NMR) technique. NMR mainly provides local structural information of protons and works most effectively on relatively small biomacromolecules. Hence structural characterization of large RNAs can be difficult for NMR alone. Electron microscopy (EM) provides global shape information of macromolecules at nanometer resolution, which should be complementary to NMR for RNA structure determination. Here we developed a new energy term in Xplor-NIH against the density map obtained by EM. We conjointly used NMR and map restraints for the structure refinement of three RNA systems-U2/U6 small-nuclear RNA, genome-packing motif (psi(CD)) 2 from Moloney murine leukemia virus, and ribosome-binding element from turnip crinkle virus. In all three systems, we showed that the incorporation of a map restraint, either experimental or generated from known PDB structure, greatly improves structural precision and accuracy. Importantly, our method does not rely on an initial model assembled from RNA duplexes, and allows full torsional freedom for each nucleotide in the torsion angle simulated annealing refinement. As increasing number of macromolecules can be characterized by both NMR and EM, the marriage between the two techniques would enable better characterization of RNA three-dimensional structures.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	SPECTROSCOPY ; DYNAMICS ; RIBOSOME ; ELEMENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000351987300144
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1063]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom Mol Phys, Natl Magnet Resonance Ctr Wuhan,Wuhan Inst Phys &, Wuhan 430071, Hubei Province, Peoples R China 2.NIH, Div Computat Biosci, Ctr Informat Technol, Bethesda, MD 20892 USA
推荐引用方式 GB/T 7714	Gong, Zhou,Schwieters, Charles D.,Tang, Chun. Conjoined Use of EM and NMR in RNA Structure Refinement[J]. PLOS ONE,2015,10(3).
APA	Gong, Zhou,Schwieters, Charles D.,&Tang, Chun.(2015).Conjoined Use of EM and NMR in RNA Structure Refinement.PLOS ONE,10(3).
MLA	Gong, Zhou,et al."Conjoined Use of EM and NMR in RNA Structure Refinement".PLOS ONE 10.3(2015).