F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells
	Ye, Yansheng 1,2; Liu, Xiaoli 1; Zhang, Zeting 1; Wu, Qiong 1; Jiang, Bin1 ; Jiang, Ling 1; Zhang, Xu 1; Liu, Maili 1; Pielak, Gary J.3; Li, Conggang 1
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2013-09-16
卷号	19 期号:38 页码:12705-12710
关键词	cytoplasmic viscosity fluorine NMR spectroscopy transient weak interactions
英文摘要	Protein mobility in living cells is vital for cell function. Both cytosolic viscosity and weak protein-protein interactions affect mobility, but examining viscosity and weak interaction effects is challenging. Herein, we demonstrate the use of (FNMR)-F-19 spectroscopy to measure cytoplasmic viscosity and to characterize nonspecific protein-protein interactions in living Escherichiacoli cells. The origins of resonance broadening in Escherichiacoli cells were also investigated. We found that sample inhomogeneity has a negligible effect on resonance broadening, the cytoplasmic viscosity is only about 2-3 times that of water, and ubiquitous transient weak protein-protein interactions in the cytosol play a significant role in governing the detection of proteins by using in-cell NMR spectroscopy.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	NUCLEAR-MAGNETIC-RESONANCE ; FLUOROTYROSINE ALKALINE-PHOSPHATASE ; MODEL-FREE APPROACH ; ESCHERICHIA-COLI ; IN-VIVO ; ROTATIONAL MOBILITY ; RELAXATION ; DIFFUSION ; CONSEQUENCES ; MACROMOLECULES
收录类别	SCI
语种	英语
WOS记录号	WOS:000324316300023
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/808]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100029, Peoples R China 3.Univ N Carolina, Dept Biochem & Biophys, Dept Chem, Chapel Hill, NC 27599 USA
推荐引用方式 GB/T 7714	Ye, Yansheng,Liu, Xiaoli,Zhang, Zeting,et al. F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells[J]. CHEMISTRY-A EUROPEAN JOURNAL,2013,19(38):12705-12710.
APA	Ye, Yansheng.,Liu, Xiaoli.,Zhang, Zeting.,Wu, Qiong.,Jiang, Bin.,...&Li, Conggang.(2013).F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.CHEMISTRY-A EUROPEAN JOURNAL,19(38),12705-12710.
MLA	Ye, Yansheng,et al."F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells".CHEMISTRY-A EUROPEAN JOURNAL 19.38(2013):12705-12710.