Metabolic profiling of a Schistosoma mansoni infection in mouse tissues using magic angle spinning-nuclear magnetic resonance spectroscopy

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Metabolic profiling of a Schistosoma mansoni infection in mouse tissues using magic angle spinning-nuclear magnetic resonance spectroscopy
	Li, Jia V.2,3; Holmes, Elaine 2; Saric, Jasmina 2,3; Keiser, Jennifer 4; Dirnhofer, Stephan 5; Utzinger, Juerg 3; Wang, Yulan 1
刊名	INTERNATIONAL JOURNAL FOR PARASITOLOGY
	2009-04-01
卷号	39 期号:5 页码:547-558
关键词	Chemometrics Magic angle spinning Metabolic profiling Mouse NMR spectroscopy Schistosomiasis Schistosoma mansoni
通讯作者	Wang, YL (reprint author), Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China.
英文摘要	In order to enhance our understanding of physiological and pathological consequences of a patent Schistosoma mansoni infection in the mouse, we examined the metabolic responses of different tissue samples recovered from the host animal using a metabolic profiling strategy. Ten female NMRI mice were infected with similar to 80 S. mansoni cercariae each, and 10 uninfected age- and sex-matched animals served as controls. At day 74 post infection (p.i.), mice were killed and jejunum, ileum, colon, liver, spleen and kidney samples were removed. We employed (1)H magic angle spinning-nuclear magnetic resonance spectroscopy to generate tissue-specific metabolic profiles. The spectral data were analyzed using multivariate modelling methods including an orthogonal signal corrected-projection to latent structure analysis and hierarchical principal component analysis to assess the differences and/or similarities in metabolic responses between infected and non-infected control mice. Most tissues obtained from S. mansoni-infected mice were characterized by high levels of amino acids, such as leucine, isoleucine, lysine, glutamine and asparagine. High levels of membrane phospholipid metabolites, including glycerophosphoryl choline and phosphoryl choline were found in the ileum, colon, liver and spleen of infected mice. Additionally, low levels of energy-related metabolites, including lipids, glucose and glycogen were observed in ileum, spleen and liver samples of infected mice. Energy-related metabolites in the jejunum, liver and renal medulla were found to be positively correlated with S. mansoni worm burden upon dissection. These findings show that a patent S. mansoni infection causes clear disruption of metabolism in a range of tissues at a molecular level, which can be interpreted in relation to the previously reported signature in a biofluid (i.e. urine), giving further evidence of the global effect of the infection. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Parasitology
研究领域[WOS]	Parasitology
关键词[WOS]	ENTERIC NERVOUS-SYSTEM ; VASOACTIVE-INTESTINAL-PEPTIDE ; HEPATOSPLENIC SCHISTOSOMIASIS ; GRANULOMATOUS INFLAMMATION ; MURINE SCHISTOSOMIASIS ; DISEASE ; MICE ; METABONOMICS ; LIVER ; RAT
收录类别	SCI
语种	英语
WOS记录号	WOS:000264221900005
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/2245]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Biomol Med, Div Surg Oncol Reprod Biol & Anaesthet, London, England 3.Swiss Trop Inst, Dept Publ Hlth & Epidemiol, CH-4002 Basel, Switzerland 4.Swiss Trop Inst, Dept Med Parasitol & Infect Biol, CH-4002 Basel, Switzerland 5.Univ Basel Hosp, Inst Pathol, CH-4031 Basel, Switzerland
推荐引用方式 GB/T 7714	Li, Jia V.,Holmes, Elaine,Saric, Jasmina,et al. Metabolic profiling of a Schistosoma mansoni infection in mouse tissues using magic angle spinning-nuclear magnetic resonance spectroscopy[J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY,2009,39(5):547-558.
APA	Li, Jia V..,Holmes, Elaine.,Saric, Jasmina.,Keiser, Jennifer.,Dirnhofer, Stephan.,...&Wang, Yulan.(2009).Metabolic profiling of a Schistosoma mansoni infection in mouse tissues using magic angle spinning-nuclear magnetic resonance spectroscopy.INTERNATIONAL JOURNAL FOR PARASITOLOGY,39(5),547-558.
MLA	Li, Jia V.,et al."Metabolic profiling of a Schistosoma mansoni infection in mouse tissues using magic angle spinning-nuclear magnetic resonance spectroscopy".INTERNATIONAL JOURNAL FOR PARASITOLOGY 39.5(2009):547-558.