Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21

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	Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21
	Li Jiang 1; Sha Yujie2
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2015-03-01
卷号	33 期号:2 页码:319-327
关键词	Antarctic bacterium Pseudoalteromonas agarase expression enzymatic characterization
英文摘要	An agar-degrading bacterium, designated as Pseudoalteromonas sp. NJ21, was isolated from an Antarctic sediment sample. The agarase gene aga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region was cloned. The gene encodes a 793-amino acids protein and was found to possess characteristic features of the Glyco_hydro_42 family. The recombinant agarase (rAga1161) was overexpressed in Escherichia coli and purified as a fusion protein. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were 30-40A degrees C and 8.0, respectively. rAga1161 was found to maintain as much as 80% of its maximum activity at 10A degrees C, which is typical of a coldadapted enzyme. The pattern of agar hydrolysis demonstrated that the enzyme is an beta-agarase, producing neoagarobiose (NA2) as the final main product. Furthermore, this work is the first proof of an agarolytic activity in Antarctic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
收录类别	SCI
语种	英语
WOS记录号	WOS:000351156800004
公开日期	2015-06-16
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/25403]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.SOA, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li Jiang,Sha Yujie. Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(2):319-327.
APA	Li Jiang,&Sha Yujie.(2015).Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(2),319-327.
MLA	Li Jiang,et al."Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.2(2015):319-327.