Biohydrogen production via the interaction of nitrogenase and anaerobic mixed-acid fermentation in marine bacteria

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Biohydrogen production via the interaction of nitrogenase and anaerobic mixed-acid fermentation in marine bacteria
	Ma, Yingchao 1,2; Huang, Aiyou 1; Zhu, Daling 3; Pan, Guanghua 3; Wang, Guangce 1
刊名	INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
	2015-01-05
卷号	40 期号:1 页码:176-183
关键词	Biohydrogen Mixed-acid fermentation Nitrogenase Marine bacteria Glycolysis Pentose phosphate pathway
英文摘要	Biohydrogen production that utilizes high-salt organic wastewater as substrate not only could treat organic waste but also could generate green energy-hydrogen. Investigation of the hydrogen-producing mechanism will lay the foundation for the industrialization of biohydrogen production. In this study, analysis revealed that mixed-acid fermentation occurred in Pantoea agglomerans. However, the amount of hydrogen that would be produced by mixed-acid fermentation (50%) was found to be less than the actual total hydrogen content in the fermenter (67.4%). Hence, a proteomic study was conducted to detect other possible pathways of hydrogen production. The results showed a nitrogenase participated in hydrogen production as a hydrogenase under anaerobic conditions. The results of both proteomics and C-13 metabolic flux ratio analysis proved the substrates consumed during hydrogen production were supplied by EMP and PPP. All of these results revealed that hydrogen production was generated by the interaction of mixed-acid fermentation and nitrogenase in the marine bacterium P. agglomerans. EMP and PPP provided pyruvate, NAD(P)H, and ATP for hydrogen production. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000348687300019
公开日期	2015-06-16
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/25355]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 256071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Tianjin Univ Sci & Technol, Tianjin Key Lab Marine Resources & Chem, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Ma, Yingchao,Huang, Aiyou,Zhu, Daling,et al. Biohydrogen production via the interaction of nitrogenase and anaerobic mixed-acid fermentation in marine bacteria[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2015,40(1):176-183.
APA	Ma, Yingchao,Huang, Aiyou,Zhu, Daling,Pan, Guanghua,&Wang, Guangce.(2015).Biohydrogen production via the interaction of nitrogenase and anaerobic mixed-acid fermentation in marine bacteria.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,40(1),176-183.
MLA	Ma, Yingchao,et al."Biohydrogen production via the interaction of nitrogenase and anaerobic mixed-acid fermentation in marine bacteria".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 40.1(2015):176-183.