A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies
	Tan, W. K.
刊名	Plant Methods
	2010
卷号	6 页码:12
ISSN号	1746-4811
中文摘要	Background: Some plants inhabiting saline environment remove salts via the salt glands embedded in the epidermal tissues. Cytological studies of salt glands will provide valuable information to our understanding of the secretory process. Previous studies on salt gland histology relied mainly on two-dimensional microscopic observations of microtome sections. Optical sectioning properties of confocal laser scanning microscope offer alternative approach for obtaining three-dimensional structural information of salt glands. Difficulty in light penetration through intact leaves and interference from neighbouring leaf cells, however, impede the acquiring of good optical salt gland sections and limit its applications in salt gland imaging. Freeing the glands from adjacent leaf tissues will allow better manipulations for three-dimensional imaging through confocal laser scanning microscopy. Results: Here, we present a simple and fast method for the isolation of individual salt glands released from the interference of neighbouring cells. About 100-200 salt glands could be isolated from just one cm(2) of Avicennia officinalis leaf within hours and microscopic visualization of isolated salt glands was made possible within a day. Using these isolated glands, confocal laser scanning microscopic techniques could be applied and better resolution salt gland images could be achieved. By making use of their intrinsic fluorescent properties, optical sections of the gland cells could be acquired without the use of fluorescent probes and the corresponding three-dimensional images constructed. Useful cytological information of the salt gland cells could also be obtained through the applications of fluorescent dyes (e.g., LysoTracker (R) Red, FM (R) 4-64, Texas Red (R)). Conclusions: The study of salt glands directly at the glandular level are made possible with the successful isolation of these specialized structures. Preparation of materials for subsequent microscopic observations of salt glands could be achieved within a day. Potential applications of confocal fluorescence microscopic techniques could also be performed using these isolated glands. Experiments designed and targeted directly at the salt glands were explored and cytological information obtained herein could be further incorporated towards the understanding of the mechanism underlying secretion in plant salt glands.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8831]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Tan, W. K.. A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies[J]. Plant Methods,2010,6:12.
APA	Tan, W. K..(2010).A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies.Plant Methods,6,12.
MLA	Tan, W. K.."A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies".Plant Methods 6(2010):12.