Extracellular secretion of phenolic substances from living brown algae | |
Shibata, T. | |
刊名 | Journal of Applied Phycology |
2006 | |
卷号 | 18期号:6页码:787-794 |
ISSN号 | 0921-8971 |
中文摘要 | The chemical structures of ultraviolet (UV)- absorbing substances secreted from the healthy living brown algae, Eisenia bicyclis and Ecklonia kurome, were demonstrated. The living activity of algal cells was critically examined using a confocal laser-scanning microscope after incorporation of fluorescein diacetate (FDA) into the cells. Using thin-layer chromatography (TLC), reversed-phase three-dimensional high-performance liquid chromatography (RP-3D-HPLC) and gas chromatography-mass spectrometry (GC-MS), it was found that the UV-absorbing substances (lambda(max) 265-270 nm) secreted from the living brown algae mainly consisted of the three monomeric bromophenols, 2,4-dibromophenol, 2,4,6-tribromophenol and dibromo-iodophenol, but not phloroglucinol or phlorotannins. The other minor compounds detected in the secretions were as follows: benzothiazole, fatty acids (14:1, 16:0 and 18:0 acids), franesol, 3-hydroxy-2,4,4-trimethylpentyl 2-methylpropanoate and squalene. Exudation of phloroglucinol and phlorotannins was ascertained to take place only after the cell death of these algae. These results indicate that, whilst the algae are alive, polymeric phlorotannins are strictly kept within the algal body, and only monomeric bromophenols are secreted into the seawater medium. |
语种 | 英语 |
公开日期 | 2015-06-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.xtbg.org.cn/handle/353005/8744] |
专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
推荐引用方式 GB/T 7714 | Shibata, T.. Extracellular secretion of phenolic substances from living brown algae[J]. Journal of Applied Phycology,2006,18(6):787-794. |
APA | Shibata, T..(2006).Extracellular secretion of phenolic substances from living brown algae.Journal of Applied Phycology,18(6),787-794. |
MLA | Shibata, T.."Extracellular secretion of phenolic substances from living brown algae".Journal of Applied Phycology 18.6(2006):787-794. |
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