Apoptosis induction and G2/M arrest of 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone from Rubia yunnanensis in human cervical cancer HeLa cells

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Apoptosis induction and G2/M arrest of 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone from Rubia yunnanensis in human cervical cancer HeLa cells
	Zeng, Guang-Zhi; Fan, Jun-Ting; Xu, Jun-Ju; Li, Yan; Tan, Ning-Hua
刊名	PHARMAZIE
	2013-04-01
卷号	68 期号:4 页码:293-299
关键词	2-Methyl-1,3,6-trihydroxy-9,10-anthraquinone (MTA) one of the major components isolated from the traditional Chinese medicine Rubia yunnanensis exhibited inhibitory activity on the proliferation of several human cancer cell lines. The results from an annexin V-FITC (fluoresein-5-isothiocyanate) apoptosis assay and DNA content analysis showed that MTA exerted cytotoxicity via apoptosis induction and G2/M cell cycle arrest in human cervical carcinoma HeLa cells. Further MTA was found to induce apoptosis of HeLa cells through the mitochondria-mediated pathway. It caused the translocation of Bax to the mitochondria and release of cytochrome c into the cytosol which caused the cleavage of caspase and poly(ADP-ribose) polymerase and finally triggered the apoptosis. Furthermore the p53/p21/Cdc2-cyclin B1 signaling was found related to the G2/M arrest caused by MTA. The over-expression of p21 and down-expression of cyclin B1 caused by MTA inactivated the Cdc2-cyclin B1 complex of G2/M checkpoint and finally caused the G2/M arrest in HeLa cells. This study demonstrated that MTA is a potential anti-cancer component of R. yunnanensis a folk anti-cancer herb used in Yunnan China.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]	Pharmacology & Pharmacy ; Chemistry
关键词[WOS]	CYTOCHROME-C RELEASE ; CYCLIC HEXAPEPTIDES ; ISOLATED-MITOCHONDRIA ; BCL-2 FAMILY ; DNA-DAMAGE ; RA-V ; ANTHRAQUINONES ; BAX ; P53 ; CONSTITUENTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000334259900011
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/19252]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Guang-Zhi,Fan, Jun-Ting,Xu, Jun-Ju,et al. Apoptosis induction and G2/M arrest of 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone from Rubia yunnanensis in human cervical cancer HeLa cells[J]. PHARMAZIE,2013,68(4):293-299.
APA	Zeng, Guang-Zhi,Fan, Jun-Ting,Xu, Jun-Ju,Li, Yan,&Tan, Ning-Hua.(2013).Apoptosis induction and G2/M arrest of 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone from Rubia yunnanensis in human cervical cancer HeLa cells.PHARMAZIE,68(4),293-299.
MLA	Zeng, Guang-Zhi,et al."Apoptosis induction and G2/M arrest of 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone from Rubia yunnanensis in human cervical cancer HeLa cells".PHARMAZIE 68.4(2013):293-299.