The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus

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	The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus
	Zhang, Tao1,2 ; Qiu, Limei 1; Sun, Zhibin 1,2; Wang, Lingling 1; Zhou, Zhi 1; Liu, Rui 1; Yue, Feng 1,2; Sun, Rui 1,2; Song, Linsheng 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2014-07-01
卷号	45 期号:1 页码:141-150
关键词	Crassostrea gigas Immune priming Phagocytosis Hemocytes regeneration Hemopoiesis
通讯作者	Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要	The increasing experimental evidences suggest that there are some forms of specific acquired immunity in invertebrates, but the underlying mechanism is not fully understood. In the present study, Pacific oyster (Crassostrea gigas) stimulated primarily by heat-killed Vibrio splendidus displayed stronger immune responses at cellular and molecular levels when they encountered the secondary challenge of live V. splendidus. The total hemocyte counts (THC) increased significantly after the primary stimulation of heat-killed V. splendidus, and it increased even higher (p < 0.01) and reached the peak earlier (at 6 h) after the secondary challenge with live V. splendidus compared with that of the primary stimulation. The number of new generated circulating hemocytes increased dramatically (p < 0.01) at 6 h after the pre-stimulated oysters received the secondary stimulation with live V. splendidus, and the phagocytic rate was also enhanced significantly (p < 0.01) at 12 h after the secondary stimulation. Meanwhile, the enhanced phagocytosis of hemocytes was highly specific for V. splendidus and they could distinguish Vibrio anguillarum, Vibrio coralliilyticus, Yarrowia lipolytica, and Micrococcus luteus efficiently. In addition, the mRNA expression of 12 candidate genes related to phagocytosis and hematopoiesis were also monitored, and the expression levels of Cgintegrin, CgPI3K (phosphatidylinositol 3-kinase), CgRho J, CgMAPKK (mitogen-activated protein kinase kinase), CgRab32, CgNADPH (nicotinamide adenine dinucleotide phosphate) oxidase, CgRunx1 and CgBMP7 (bone morphogenetic protein 7) in the hemocytes of pre-stimulated oysters after the secondary stimulation of V. splendidus were higher (p < 0.01) than that after the primary stimulation, but there was no statistically significant changes for the genes of CgPKC (protein kinase C), CgMyosin, CgActin, and CgGATA 3. These results collectively suggested that the primary stimulation of V. splendidus led to immune priming in oyster with specifically enhanced phagocytosis and rapidly promoted regeneration of circulating hemocytes when the primed oysters encountered the secondary challenge with V. splendidus. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Immunology; Zoology
收录类别	SCI
语种	英语
WOS记录号	WOS:000335624800015
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24186]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Tao,Qiu, Limei,Sun, Zhibin,et al. The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,45(1):141-150.
APA	Zhang, Tao.,Qiu, Limei.,Sun, Zhibin.,Wang, Lingling.,Zhou, Zhi.,...&Song, Linsheng.(2014).The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,45(1),141-150.
MLA	Zhang, Tao,et al."The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 45.1(2014):141-150.