Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515
	Wu, Songcui1,2 ; Zhang, Baoyu 1; Huang, Aiyou 1; Huan, Li1,2 ; He, Linwen 1; Lin, Apeng 1; Niu, Jianfeng 1; Wang, Guangce 1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2014-08-01
卷号	26 期号:4 页码:1659-1668
关键词	Marine microalga BODIPY 505/515 Nile red Neutral lipid Triacylglycerol Biodiesel
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao 266071, Peoples R China.
英文摘要	Diatoms and dinoflagellates not only have extensive distribution and a huge biomass in marine ecosystems, but also have high lipid accumulation in nature or after physiological and genetic modification, which indicates that these organisms may be optimal candidate algal strains for biodiesel production. In this study, we determined the content of intracellular neutral lipids (triacylglycerol [TAG]) in the dinoflagellate Prorocentrum micans and in the diatom Phaeodactylum tricornutum using NR and BODIPY 505/515 staining. The freshwater green alga Scenedesmus obliquus was used as a control. Optimum concentrations of 1.000 and 1.500 mu g mL(-1) were determined for neutral lipid Nile red (NR) staining in P. micans and P. tricornutum. Unlike NR staining, the optimal concentrations of BODIPY 505/515 staining in P. micans and P. tricornutum were lower, at 0.100 and 0.075 mu g mL(-1), respectively. High correlation coefficients of R (2) = 0.990 and R (2) = 0.989 were obtained for P. micans and P. tricornutum intracellular neutral lipid content and the relative fluorescence intensity with NR staining, while the reference alga, S. obliquus, had a relatively low correlation coefficient of R (2) = 0.908 when stained with NR. The neutral lipid content determined by thin-layer chromatography-flame ionization detector matched the analytical data from fluorescence measurements. These results indicated that NR and BODIPY 505/515 staining can be used as an excellent high-throughput approach to screen marine diatoms and dinoflagellates.
学科主题	Biotechnology & Applied Microbiology; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000340101100005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24101]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wu, Songcui,Zhang, Baoyu,Huang, Aiyou,et al. Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(4):1659-1668.
APA	Wu, Songcui.,Zhang, Baoyu.,Huang, Aiyou.,Huan, Li.,He, Linwen.,...&Wang, Guangce.(2014).Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515.JOURNAL OF APPLIED PHYCOLOGY,26(4),1659-1668.
MLA	Wu, Songcui,et al."Detection of intracellular neutral lipid content in the marine microalgae Prorocentrum micans and Phaeodactylum tricornutum using Nile red and BODIPY 505/515".JOURNAL OF APPLIED PHYCOLOGY 26.4(2014):1659-1668.