Characterization of the AOX gene and cyanide-resistant respiration in Pyropia haitanensis (Rhodophyta)

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	Characterization of the AOX gene and cyanide-resistant respiration in Pyropia haitanensis (Rhodophyta)
	Zhang, Bao Yu 1; Zhu, Da Ling 2; Wang, Guang Ce 1; Peng, Guang 1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2014-12-01
卷号	26 期号:6 页码:2425-2433
关键词	Alternative oxidase Pyropia haitanensis mRNA expression Alternative respiration pathway Noninvasive microsensing system
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要	In order to characterize the structure and function of the alternative oxidase (AOX) gene in Pyropia haitanensis, full-length complementary DNA (cDNA) of the locus (denoted as PhAOX) was obtained by in silico cloning, rapid amplification of cDNA ends, and reverse transcription PCR. The PhAOX sequence was 3,192 bp including a 5'-untranslated region (UTR) of 1,484 bp, a 3'-UTR of 274 bp, and an open reading frame of 1,434 bp predicted to encode a protein of 477 amino acids with a molecular mass of 50.47 kDa and a putative isoelectric point of 9.75. PhAOX is predicted to be a precursor protein with mitochondrial-targeting peptides. The deduced amino acid sequence of PhAOX was most similar to PyAOX from Porphyra (= Pyropia) yezoensis (77 % identity); however, gene sequences from PhAOX and PyAOX showed obvious divergence. PhAOX had a 315-bp intron but PyAOX had no intron. Expression of mRNA in filamentous thalli and the foliose thallus, which are different life cycle stages, was examined by real-time fluorescent quantitative PCR (qPCR). The highest PhAOX expression was in filamentous thalli and was about 10 times the expression in the foliose thallus. We showed that an alternative respiration pathway also existed in P. haitanensis using a noninvasive microsensing system. The contribution of the alternative pathway to total respiration in filamentous thalli was greater than the contribution in the foliose thallus. This result was consistent with the level of PhAOX gene expression observed in different life cycle stages.
学科主题	Biotechnology & Applied Microbiology; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000344350700017
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24086]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Bao Yu,Zhu, Da Ling,Wang, Guang Ce,et al. Characterization of the AOX gene and cyanide-resistant respiration in Pyropia haitanensis (Rhodophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(6):2425-2433.
APA	Zhang, Bao Yu,Zhu, Da Ling,Wang, Guang Ce,&Peng, Guang.(2014).Characterization of the AOX gene and cyanide-resistant respiration in Pyropia haitanensis (Rhodophyta).JOURNAL OF APPLIED PHYCOLOGY,26(6),2425-2433.
MLA	Zhang, Bao Yu,et al."Characterization of the AOX gene and cyanide-resistant respiration in Pyropia haitanensis (Rhodophyta)".JOURNAL OF APPLIED PHYCOLOGY 26.6(2014):2425-2433.