Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio) | |
Wang, Qian1,2; Tan, Xungang1; Jiao, Shuang1; You, Feng1; Zhang, Pei-Jun1 | |
刊名 | PLOS ONE |
2014-07-24 | |
卷号 | 9期号:7页码:e102492 |
通讯作者 | Tan, XG (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China. |
英文摘要 | Low temperatures may cause severe growth inhibition and mortality in fish. In order to understand the mechanism of cold tolerance, a transgenic zebrafish Tg (smyd1:m3ck) model was established to study the effect of energy homeostasis during cold stress. The muscle-specific promoter Smyd1 was used to express the carp muscle form III of creatine kinase (M3-CK), which maintained enzymatic activity at a relatively low temperature, in zebrafish skeletal muscle. In situ hybridization showed that M3-CK was expressed strongly in the skeletal muscle. When exposed to 13 degrees C, Tg (smyd1:m3ck) fish maintained their swimming behavior, while the wild-type could not. Energy measurements showed that the concentration of ATP increased in Tg (smyd1:m3ck) versus wild-type fish at 28 degrees C. After 2 h at 13 degrees C, ATP concentrations were 2.16-fold higher in Tg (smyd1:m3ck) than in wild-type (P<0.05). At 13 degrees C, the ATP concentration in Tg (smyd1:m3ck) fish and wild-type fish was 63.3% and 20.0%, respectively, of that in wild-type fish at 28 degrees C. Microarray analysis revealed differential expression of 1249 transcripts in Tg (smyd1:m3ck) versus wild-type fish under cold stress. Biological processes that were significantly overrepresented in this group included circadian rhythm, energy metabolism, lipid transport, and metabolism. These results are clues to understanding the mechanisms underlying temperature acclimation in fish. |
学科主题 | Science & Technology - Other Topics |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000341354800022 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/24071] |
专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋地质与环境重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Qian,Tan, Xungang,Jiao, Shuang,et al. Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio)[J]. PLOS ONE,2014,9(7):e102492. |
APA | Wang, Qian,Tan, Xungang,Jiao, Shuang,You, Feng,&Zhang, Pei-Jun.(2014).Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio).PLOS ONE,9(7),e102492. |
MLA | Wang, Qian,et al."Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio)".PLOS ONE 9.7(2014):e102492. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论