Cold-active antibacterial and antifungal activities and antibiotic resistance of bacteria isolated from an alpine hydrocarbon-contaminated industrial site

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	Cold-active antibacterial and antifungal activities and antibiotic resistance of bacteria isolated from an alpine hydrocarbon-contaminated industrial site
	Hemala, Lydia 1; Zhang, Dechao 1,2; Margesin, Rosa 1
刊名	RESEARCH IN MICROBIOLOGY
	2014-07-01
卷号	165 期号:6 页码:447-456
关键词	Antibiotics Antibacterial Antifungal Cold-active Hydrocarbons
通讯作者	Margesin, R (reprint author), Univ Innsbruck, Inst Microbiol, Technikerstr 25, A-6020 Innsbruck, Austria.
英文摘要	Selection pressure in hydrocarbon-contaminated soils may lead not only to increased microbial resistance to antibiotics, but also to increased capacity of the soil indigenous population to produce antimicrobial compounds. Therefore, we studied the antibiotic resistance pattern and antibacterial and/or antifungal activities of 47 bacterial strains isolated from an industrial alpine site heavily polluted with petroleum hydrocarbons. Resistance to penicillin was more widespread (49%) than resistance to chloramphenicol or rifampicin (28%) or streptomycin (26%). Only 9% of the strains were resistant to tetracycline. The ability to produce cold-active (10 degrees C) antimicrobial compounds was tested by using human pathogenic bacteria (Escherichia coli, Shigella flexneri, Salmonella enterica, Pseudomonas aeruginosa, Staphylococcus aureus) and yeasts (Candida albicans, Cryptococcus neoformans) as indicator microorganisms. About two-thirds of the 47 tested strains produced compounds that inhibited growth of at least one indicator microorganism. Six strains inhibited growth of both bacteria and yeast indicators; 12 and 16 strains showed either antibacterial or antifungal activity, respectively. The most versatile bacteria with regard to multiple antibiotic resistance and antimicrobial activity belonged to Actinobacteria or Gammaproteobacteria. The antimicrobial compounds produced by three Pseudomonas spp. and two Serratia spp. strains were characterized in more detail by TLC and HPLC. Depending on the sensitivity of growth inhibition to enzymes, the compounds produced by the three pseudomonads contained a proteinaceous component. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
学科主题	Microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000347626000006
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/23768]
专题	海洋研究所_海洋生物分类与系统演化实验室
作者单位	1.Univ Innsbruck, Inst Microbiol, A-6020 Innsbruck, Austria 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Hemala, Lydia,Zhang, Dechao,Margesin, Rosa. Cold-active antibacterial and antifungal activities and antibiotic resistance of bacteria isolated from an alpine hydrocarbon-contaminated industrial site[J]. RESEARCH IN MICROBIOLOGY,2014,165(6):447-456.
APA	Hemala, Lydia,Zhang, Dechao,&Margesin, Rosa.(2014).Cold-active antibacterial and antifungal activities and antibiotic resistance of bacteria isolated from an alpine hydrocarbon-contaminated industrial site.RESEARCH IN MICROBIOLOGY,165(6),447-456.
MLA	Hemala, Lydia,et al."Cold-active antibacterial and antifungal activities and antibiotic resistance of bacteria isolated from an alpine hydrocarbon-contaminated industrial site".RESEARCH IN MICROBIOLOGY 165.6(2014):447-456.