Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino
	Yang Hongsheng
刊名	JOURNAL OF OCEAN UNIVERSITY OF CHINA
	2014
卷号	13 期号:6 页码:1033-1042
关键词	Apostichopus japonicus sezparation gene expression profiling RNA-seq real-time PCR
通讯作者	Yang, HS (reprint author), Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China.
英文摘要	Sea cucumber (Apostichopus japonicus) is one of the most important aquaculture animals in China. Usually its normal body color is black that fits its living environment. The juvenile individuals obtained by crossing albino sea cucumber segregated in body color. To document the transcriptome difference between albino associating sea cucumber and the control, we sequenced their transcriptomes with RNA-seq. Approximately, 4.790 million (M) and 4.884 M reads, 200 nt in length, were generated from the body wall of albino associating sea cucumber and the control, respectively, from them, 9550 (46.81%) putative genes were identified. In total, 583 genes were found to express differentially between albino associating sea cucumber and the control. Of these differentially expressed genes (DEGs), 4.8% changed more than five-folds. The expression levels of eight DEGs were confirmed with real-time PCR. The changing trend of these DEGs detected with real-time PCR agreed well with that detected with RNA-seq, although the change degree of some DEGs was different. Four significantly enriched pathways were identified for DEGs, which included phagocytosis, Staphylococcus aureus infection, ECM-receptor interaction and focal adhesion. These pathways were helpful for understanding the physiological difference between albino associating sea cucumber and the control.
学科主题	Oceanography
收录类别	SCI
语种	英语
WOS记录号	WOS:000344788700020
公开日期	2015-06-11
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24036]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Yang Hongsheng
推荐引用方式 GB/T 7714	Yang Hongsheng. Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2014,13(6):1033-1042.
APA	Yang Hongsheng.(2014).Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino.JOURNAL OF OCEAN UNIVERSITY OF CHINA,13(6),1033-1042.
MLA	Yang Hongsheng."Profiling and comparison of color body wall transcriptome of normal juvenile sea cucumber (Apostichopus japonicus) and those produced by crossing albino".JOURNAL OF OCEAN UNIVERSITY OF CHINA 13.6(2014):1033-1042.