Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1

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	Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
	Wang, Guoshan 1; Yu, Zhigang 2,3; Zhen, Yu 4,5; Mi, Tiezhu 4,5; Shi, Yan 5; Wang, Jianyan 1; Wang, Minxiao 6; Sun, Song6
刊名	PLOS ONE
	2014-06-13
卷号	9 期号:6 页码:e100057
通讯作者	Zhen, Y (reprint author), Minist Educ, Key Lab Marine Environm & Ecol, Qingdao, Shandong, Peoples R China.
英文摘要	The maintenance of physiological oxygen homeostasis is mediated by hypoxia-inducible factor (HIF), a key transcriptional factor of the PHD-HIF system in all metazoans. However, the molecular evolutionary origin of this central physiological regulatory system is not well characterized. As the earliest eumetazoans, Cnidarians can be served as an interesting model for exploring the HIF system from an evolutionary perspective. We identified the complete cDNA sequence of HIF-1 alpha (ASHIF) from the Aurelia sp.1, and the predicted HIF-1 alpha protein (pASHIF) was comprised of 674 amino acids originating from 2,025 bp nucleotides. A Pairwise comparison revealed that pASHIF not only possessed conserved basic helix-loop-helix (bHLH) and Per-Arnt-Sim (PAS) domains but also contained the oxygen dependent degradation (ODD) and the C-terminal transactivation domains (C-TAD), the key domains for hypoxia regulation. As indicated by sequence analysis, the ASHIF gene contains 8 exons interrupted by 7 introns. Western blot analysis indicated that pASHIF that existed in the polyps and medusa of Aurelia. sp.1 was more stable for a hypoxic response than normoxia.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000338278100112
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24028]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Shandong, Peoples R China 2.Minist Educ, Key Lab Marine Chem Theory & Engn, Qingdao, Shandong, Peoples R China 3.Ocean Univ China, Coll Chem & Chem Engn, Qingdao, Shandong, Peoples R China 4.Minist Educ, Key Lab Marine Environm & Ecol, Qingdao, Shandong, Peoples R China 5.Ocean Univ China, Coll Environm Sci & Engn, Qingdao, Shandong, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Guoshan,Yu, Zhigang,Zhen, Yu,et al. Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1[J]. PLOS ONE,2014,9(6):e100057.
APA	Wang, Guoshan.,Yu, Zhigang.,Zhen, Yu.,Mi, Tiezhu.,Shi, Yan.,...&Sun, Song.(2014).Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1.PLOS ONE,9(6),e100057.
MLA	Wang, Guoshan,et al."Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1".PLOS ONE 9.6(2014):e100057.