A facile approach to construct versatile signal amplification system for bacterial detection

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	A facile approach to construct versatile signal amplification system for bacterial detection
	Qi, Peng1,2 ; Zhang, Dun 1; Wan, Yi 1; Lv, Dandan 1,2
刊名	TALANTA
	2014-01-15
卷号	118 页码:333-338
关键词	Signal amplification Bacterial detection Dopamine Enhanced polymerization
通讯作者	Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	In this work, a facile approach to design versatile signal amplification system for bacterial detection has been presented. Bio-recognition elements and signaling molecules can be immobilized on the surface of Fe3O4@MnO2 nanomaterials with the help of bioinspired polydopamine (PDA). Fe3O4@MnO2 nanoplates were chosen as carrier for bio-recognizing and signaling molecules because this kind of nanomaterial was superparamagnetic and the existence of MnO2 could enhance the polymerization of dopamine due to its strong oxidative ability. This nanocomposite system was versatile because PDA around Fe3O4@MnO2 nanoplates provided a stable and convenient platform for immobilization of biological and chemical materials, and various kinds of bio-recognizing and signaling molecules could be immobilized by reaction with pendant amino groups of dopamine to meet different detection requirements. Since a substantial amount of signaling molecules were immobilized on the surface of the nanocomposites, so the sensitivity of detection would be improved when the prepared nanocomposites were selectively conjugated with target pathogen. In the experimental section, a sandwich-type electrochemical biosensor was developed to verify the amplified bacterial detection sensitivity. Concanavalin A (conA) and ferrocene (Fc) were chosen as bio-recognition elements and signaling molecules for detection of Desulforibrio caledoiensis, respectively. The conA and Fc modified nanocomposites were conjugated on electrode by the selective recognition between conA and target bacteria, and the bacterial population was obtained by quantification of the electrochemical signal of Fc moieties. The experimental results showed that the detection sensitivity for D. caledoiensis was improved by taking advantage of this signal amplification system. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	Chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000329553600043
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/23854]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Qi, Peng,Zhang, Dun,Wan, Yi,et al. A facile approach to construct versatile signal amplification system for bacterial detection[J]. TALANTA,2014,118:333-338.
APA	Qi, Peng,Zhang, Dun,Wan, Yi,&Lv, Dandan.(2014).A facile approach to construct versatile signal amplification system for bacterial detection.TALANTA,118,333-338.
MLA	Qi, Peng,et al."A facile approach to construct versatile signal amplification system for bacterial detection".TALANTA 118(2014):333-338.