PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine
	Huang, Qingrui 1,4; Li, Dongxia 1; Kang, Aijun 3; An, Wenqi 2; Fan, Bei 2; Ma, Xiaowei 2; Ma, Guanghui 1; Su, Zhiguo 1; Hu, Tao 1
刊名	JOURNAL OF CONTROLLED RELEASE
	2013-11-28
卷号	172 期号:1 页码:382-389
关键词	Neisseria meningitidis Conjugate vaccine Spacer arm Polyethylene glycol Immunogenicity
英文摘要	Neisseria meningitidis is a life-threatening pathogen that causes meningitis and other clinical manifestations. As a key virulence determinant, meningococcal capsular polysaccharide (PS) can be used to prevent meningococcal diseases. Conjugation of PS to carrier protein can significantly improve the immunogenicity of PS and induce memory response in infants and young children. However, the conjugate vaccine may suffer from steric shielding of antigenic PS epitopes by carrier protein. Here, a heterobifunctional polyethylene glycol (PEG) was used as a spacer arm to conjugate meningococcal group Y capsular PS with tetanus toxoid (TT). PEG can avoid self-crosslink of PS and increase the PS/TT ratio of the vaccine. Significant structural change in TT and PS was not observed upon conjugation. As compared to the vaccine without PEG, immunization with the vaccine using PEG as the spacer arm led to a 3.0-fold increase in the PS-specific IgG titers and a prolonged immune persistence. Paradoxically, PEG, a non-immunogenic hydrophilic polymer has been widely used to couple therapeutic protein for increasing its circulatory time and decreasing its immunogenicity. Presumably, PEG can fully decrease the steric shielding effect of TT on antigenic epitopes of PS and suppress the immunogenicity of TT. In addition, PEG can prolong the immune persistence of the conjugate vaccine and improve its ability to elicit cellular immunity. Thus, PEG can be used as a spacer arm to develop more effective PS conjugate vaccine for prevention of bacterial infection. (C) 2013 Elsevier B. V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]	Chemistry ; Pharmacology & Pharmacy
关键词[WOS]	PEGYLATED HEMOGLOBIN ; IN-VIVO ; DISEASE ; STAPHYLOKINASE ; THIOLATION ; ANTIBODIES ; TOLERANCE ; ANTIGEN ; SAFETY
收录类别	SCI
语种	英语
WOS记录号	WOS:000327601200043
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13662]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Hualan Biol Engn Inc, Xinxiang 453003, Henan Province, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Dept Lab Anim Sci, Beijing 100191, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Huang, Qingrui,Li, Dongxia,Kang, Aijun,et al. PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine[J]. JOURNAL OF CONTROLLED RELEASE,2013,172(1):382-389.
APA	Huang, Qingrui.,Li, Dongxia.,Kang, Aijun.,An, Wenqi.,Fan, Bei.,...&Hu, Tao.(2013).PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine.JOURNAL OF CONTROLLED RELEASE,172(1),382-389.
MLA	Huang, Qingrui,et al."PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine".JOURNAL OF CONTROLLED RELEASE 172.1(2013):382-389.