Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay

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	Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay
	Yang, Mingzhu 1; Guan, Yueping 1; Yang, Yu 2; Xia, Tingting 1; Xiong, Wubin 1; Wang, Ning 3; Guo, Chen 3
刊名	JOURNAL OF COLLOID AND INTERFACE SCIENCE
	2013-09-01
卷号	405 期号:0 页码:291-295
关键词	Magnetic nanoparticle Peroxidase-like activity Immunoassay
英文摘要	Amino-functionalized Fe3O4 magnetic nanoparticles with high peroxidase-like activity (MNPs-NH2) are reported in this work. The peroxidase-like activity is approximately the same as naked Fe3O4 magnetic nanoparticles (MNPs). MNPs-NH2 displayed much better thermal stability and pH tolerance than horse-radish peroxidase (HRP). The optimal pH and temperature are approximately pH 4 and 40 degrees C, respectively. We showed that the MNPs-NH2 were successfully applied in a double-antibody sandwich "enzyme"-linked immunosorbent assay to replace of HRP, in which MNPs-NH2 were not only separation carriers, but also detection indicator. (C) 2013 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Physical
研究领域[WOS]	Chemistry
关键词[WOS]	FERROMAGNETIC NANOPARTICLES ; PARTICLES ; DELIVERY ; PROGRESS
收录类别	SCI
语种	英语
WOS记录号	WOS:000321598400039
公开日期	2015-05-27
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/13598]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.China Acad Inspect & Quarantine, Beijing 100123, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Yang, Mingzhu,Guan, Yueping,Yang, Yu,et al. Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2013,405(0):291-295.
APA	Yang, Mingzhu.,Guan, Yueping.,Yang, Yu.,Xia, Tingting.,Xiong, Wubin.,...&Guo, Chen.(2013).Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay.JOURNAL OF COLLOID AND INTERFACE SCIENCE,405(0),291-295.
MLA	Yang, Mingzhu,et al."Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay".JOURNAL OF COLLOID AND INTERFACE SCIENCE 405.0(2013):291-295.