Dynamics of tRNA translocation, mRNA translocation and tRNA dissociation during ribosome translation through mRNA secondary structures

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	Dynamics of tRNA translocation, mRNA translocation and tRNA dissociation during ribosome translation through mRNA secondary structures
	Xie, P
刊名	EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
	2014
卷号	43 期号:6-7 页码:229
关键词	tRNA dissociation tRNA translocation mRNA translocation Translation Hybrid state
ISSN号	0175-7571
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China.
中文摘要	The ribosome can translate through the duplex region or secondary structure of mRNA. Recent single-molecule experimental data showed that downstream mRNA secondary structures have more sensitive effects on deacylated tRNA dissociation from the E site than on tRNA translocation in the 50S subunit. However, it is unclear how the downstream mRNA secondary structure can affect the tRNA dissociation from the E site, which is distant from the secondary structure. Here, based on our proposed ribosomal translocation model, we theoretically study the dynamics of tRNA translocation in the 50S subunit, mRNA translocation and tRNA dissociation, giving quantitative explanations of the single-molecule experimental data. It is shown that the effect of the downstream mRNA secondary structure on tRNA dissociation is via the effect on mRNA translocation, while the mRNA secondary structure has no effect on the rate of deacylated tRNA dissociation from the posttranslocation state. The slow mRNA translocation, which results in slow tRNA dissociation, derives from the occurrence of the futile transition, which is induced by the energy barrier from base pair unwinding to resist the forward translocation. The reduced translation rate through the mRNA secondary structure is induced by the slow mRNA translocation rather than the slow tRNA dissociation.
资助信息	National Natural Science Foundation of China [11374352]
语种	英语
公开日期	2015-04-14
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/59073]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Xie, P. Dynamics of tRNA translocation, mRNA translocation and tRNA dissociation during ribosome translation through mRNA secondary structures[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,2014,43(6-7):229.
APA	Xie, P.(2014).Dynamics of tRNA translocation, mRNA translocation and tRNA dissociation during ribosome translation through mRNA secondary structures.EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,43(6-7),229.
MLA	Xie, P."Dynamics of tRNA translocation, mRNA translocation and tRNA dissociation during ribosome translation through mRNA secondary structures".EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 43.6-7(2014):229.