Prospects Application of Polypyrrole-Based Immunosensor to Porphyromonas Gingivalis Quantification in Subgingival Plaque Samples

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	Prospects Application of Polypyrrole-Based Immunosensor to Porphyromonas Gingivalis Quantification in Subgingival Plaque Samples
	Pei, ZH ; Quan, BG ; Niu, ZY ; Shi, SG ; Shi, L ; Tang, CH ; Liu, Y
刊名	CLINICAL LABORATORY
	2014
卷号	60 期号:4 页码:525
关键词	Porphyromonas gingivalis impedance polypyrrole periodontitis immunosensor
ISSN号	1433-6510
通讯作者	Niu, ZY (reprint author), 306th Hosp PLA, Dept Stomatol, Beijing 100101, Peoples R China.
中文摘要	Background: Periodontitis still poses a serious threat to oral and systemic health condition of humans. The proportion of the main pathogenic bacteria change in localized sites was associated with the initiation of the disease process. However, the limitations of microbiological diagnostic aids rendered the diagnosis of active periodontitis status point-of-care or chair-side based on microbiological data difficult. Methods: Porphyromonas gingivalis, a major putative etiological agent in the initiation and progression of chronic periodontitis, was used as the experimental subject. An immunosensor based on polypyrrole-coated interdigitated array microelectrodes was developed to quantify Porplzyromonas gingivalis in pure culture, gingival crevicular fluid and saliva samples. The regression equation for the normalized impedance change (NIC) versus Porphyromonas gingivalis concentration (C) was measured. The correlation between results of the immunosensor and quantitative real-time PCR method in quantifying Porphyromonas gingivalis in subgingival plaque samples was evaluated. Results: Results of the study revealed that the lowest detection limits of the immunosensor was 1.9 x 10(4), 2.7 x 10(5), and 2.7 x 10(6) cells/mL in pure culture, gingival crevicular fluid, and saliva samples respectively. The values determined using the immunosensor strongly correlated with those obtained using quantitative real-time PCR method (R-2 = 0.91, p < 0.05). The immunosensor did not require any labels and amplification steps, and the total detection time from sampling to measurement was less than one hour. Conclusions: The immunosensor developed in the present study offered some insight into monitoring the change in the number of periodontal bacteria chair-side during routine clinical practice.
资助信息	Research Fund of Capital Medical Development [2009-3065]; Applied Research Programs of Capital Clinical Characteristics Foundation [D101100050010053]; National Natural Science Foundation of China [51103175]
语种	英语
公开日期	2015-04-14
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/59052]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Pei, ZH,Quan, BG,Niu, ZY,et al. Prospects Application of Polypyrrole-Based Immunosensor to Porphyromonas Gingivalis Quantification in Subgingival Plaque Samples[J]. CLINICAL LABORATORY,2014,60(4):525.
APA	Pei, ZH.,Quan, BG.,Niu, ZY.,Shi, SG.,Shi, L.,...&Liu, Y.(2014).Prospects Application of Polypyrrole-Based Immunosensor to Porphyromonas Gingivalis Quantification in Subgingival Plaque Samples.CLINICAL LABORATORY,60(4),525.
MLA	Pei, ZH,et al."Prospects Application of Polypyrrole-Based Immunosensor to Porphyromonas Gingivalis Quantification in Subgingival Plaque Samples".CLINICAL LABORATORY 60.4(2014):525.