Detection and analysis of DNA recapture through a solid-state nanopore

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	Detection and analysis of DNA recapture through a solid-state nanopore
	Hu, Y ; Zhou, Z ; Shan, XY ; Lu, XH
刊名	CHINESE SCIENCE BULLETIN
	2014
卷号	59 期号:35 页码:4953
关键词	DNA recapture Solid-state nanopore Membrane capacitance COMSOL simulation Prohibited time
ISSN号	1001-6538
通讯作者	Lu, XH (reprint author), Chinese Acad Sci, Inst Phys, Beijing 100190, Peoples R China.
中文摘要	Motion control of a single molecule through a solid-state nanopore offers a new perspective on detecting and analyzing single biomolecules. Repeat recapture of a single DNA molecule reveals the dynamics in DNA translocation through a nanopore and may significantly increase the signal-to-noise ratio for DNA base distinguishing. However, the transient current at the moment of voltage reversal prevents the observation of instantly recaptured molecules and invalidates the continuous DNA ping-pong control. We performed and analyzed the DNA translocation and recapture experiment in a silicon nitride solid-state nanopore. Numerical calculation of molecular motion clearly shows the recapture dynamics with different delay times. The prohibited time when the data acquisition system is saturated by the transient current is derived by equivalent circuit analysis and finite element simulation. The COMSOL simulation reveals that the membrane capacitance plays an important role in determining the electric field distribution during the charging process. As a result of the transient charging process, a non-constant driving force pulls the DNA back to nanopores faster than theoretically predicted. The observed long time constant in the transient current trace is explained by the dielectric absorption of the membrane capacitor.
资助信息	National Basic Research Program of China [2012CB933002]; Chinese Academy of Sciences [XDB07030100]; State Key Laboratory of Low-Dimensional Quantum Physics [KF201201]; Hundred Talent Program of Chinese Academy of Sciences
语种	英语
公开日期	2015-04-14
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/59045]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Hu, Y,Zhou, Z,Shan, XY,et al. Detection and analysis of DNA recapture through a solid-state nanopore[J]. CHINESE SCIENCE BULLETIN,2014,59(35):4953.
APA	Hu, Y,Zhou, Z,Shan, XY,&Lu, XH.(2014).Detection and analysis of DNA recapture through a solid-state nanopore.CHINESE SCIENCE BULLETIN,59(35),4953.
MLA	Hu, Y,et al."Detection and analysis of DNA recapture through a solid-state nanopore".CHINESE SCIENCE BULLETIN 59.35(2014):4953.