An explanation of biphasic characters of mRNA translocation in the ribosome

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	An explanation of biphasic characters of mRNA translocation in the ribosome
	Xie, P
刊名	BIOSYSTEMS
	2014
卷号	118 页码:1
关键词	Ribosome Translocation Hybrid state Translation Protein synthesis
ISSN号	0303-2647
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China.
中文摘要	Translocation is an essential step in the elongation cycle of protein synthesis in which mRNA that is coupled with tRNAs by codon-anticodon interaction is moved through the ribosome. It has been well documented that the kinetics of mRNA translocation generally shows biphasic character. However, the physical basis of the phenomenon is unclear. Here, to explain the phenomenon we consider two models. In one model (Model I), besides the classical non-rotated and rotated conformations of the ribosome there also exists an intermediate conformation between the two classical conformations. The mRNA translocation occurs via proceeding from the rotated (hybrid) pretranslocation to intermediate to nonrotated posttranslocation state. In another model (Model II), only the classical non-rotated and rotated conformations are considered. Before EF-G binding, the ribosomal complex is in either the classical nonrotated or rotated (hybrid) pretranslocation state, with the equilibrium with each other. EF-G can bind to both states and then the mRNA translocation occurs via proceeding either directly from the hybrid to non-rotated posttranslocation state or from the non-rotated pretranslocation to hybrid to non-rotated posttranslocation state. Analytical studies showed that Model I is unable to explain the biphasic character of mRNA translocation. By contrast, Model II can not only provide a good explanation of the biphasic character of mRNA translocation but also explain the kinetics of the reverse ribosomal rotation from the rotated to non-rotated conformation, which can be fit to a single exponential. Thus, Model II could be the appropriate one for the kinetic pathway of mRNA translocation. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
资助信息	National Natural Science Foundation of China [11374352]
语种	英语
公开日期	2015-04-14
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/58938]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Xie, P. An explanation of biphasic characters of mRNA translocation in the ribosome[J]. BIOSYSTEMS,2014,118:1.
APA	Xie, P.(2014).An explanation of biphasic characters of mRNA translocation in the ribosome.BIOSYSTEMS,118,1.
MLA	Xie, P."An explanation of biphasic characters of mRNA translocation in the ribosome".BIOSYSTEMS 118(2014):1.