CMOS-Compatible Silicon Nanowire Field-Effect Transistors for Ultrasensitive and Label-Free MicroRNAs Sensing

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	CMOS-Compatible Silicon Nanowire Field-Effect Transistors for Ultrasensitive and Label-Free MicroRNAs Sensing
	Lu, N ; Gao, AR ; Dai, PF ; Song, SP ; Fan, CH ; Wang, YL ; Li, T
刊名	SMALL
	2014
卷号	10 期号:10 页码:2022—2028
关键词	ELECTRICAL DETECTION CANCER PATIENTS EXPRESSION BIOSENSORS RNAS NANOSENSORS ELEGANS GENES ACID DNA
ISSN号	1613-6810
通讯作者	fchh@sinap.ac.cn ; ylwang@mail.sim.ac.cn ; tli@mail.sim.ac.cn
英文摘要	MicroRNAs (miRNAs) have been regarded as promising biomarkers for the diagnosis and prognosis of early-stage cancer as their expression levels are associated with different types of human cancers. However, it is a challenge to produce low-cost miRNA sensors, as well as retain a high sensitivity, both of which are essential factors that must be considered in fabricating nanoscale biosensors and in future biomedical applications. To address such challenges, we develop a complementary metal oxide semiconductor (CMOS)-compatible SiNW-FET biosensor fabricated by an anisotropic wet etching technology with self-limitation which provides a much lower manufacturing cost and an ultrahigh sensitivity. This nanosensor shows a rapid (< 1 minute) detection of miR-21 and miR-205, with a low limit of detection (LOD) of 1 zeptomole (ca. 600 copies), as well as an excellent discrimination for single-nucleotide mismatched sequences of tumor-associated miRNAs. To investigate its applicability in real settings, we have detected miRNAs in total RNA extracted from lung cancer cells as well as human serum samples using the nanosensors, which demonstrates their potential use in identifying clinical samples for early diagnosis of cancer.
收录类别	SCI
语种	英语
WOS记录号	WOS:000336611000019
公开日期	2015-03-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/14042]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Lu, N,Gao, AR,Dai, PF,et al. CMOS-Compatible Silicon Nanowire Field-Effect Transistors for Ultrasensitive and Label-Free MicroRNAs Sensing[J]. SMALL,2014,10(10):2022—2028.
APA	Lu, N.,Gao, AR.,Dai, PF.,Song, SP.,Fan, CH.,...&Li, T.(2014).CMOS-Compatible Silicon Nanowire Field-Effect Transistors for Ultrasensitive and Label-Free MicroRNAs Sensing.SMALL,10(10),2022—2028.
MLA	Lu, N,et al."CMOS-Compatible Silicon Nanowire Field-Effect Transistors for Ultrasensitive and Label-Free MicroRNAs Sensing".SMALL 10.10(2014):2022—2028.