Optimized culture condition for enhancing lytic performance of waste activated sludge by Geobacillus sp G1

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Optimized culture condition for enhancing lytic performance of waste activated sludge by Geobacillus sp G1
	Liu, Wenzong
刊名	WATER SCIENCE AND TECHNOLOGY
	2014
卷号	70 期号:2 页码:200-208
关键词	Geobacillus lytic performance response surface methodology (RSM) uniform design (UD) waste activated sludge (WAS)
ISSN号	0273-1223
中文摘要	Hydrolysis is known as the rate-limiting step during waste activated sludge (WAS) digestion. The optimization of the culture conditions of Geobacillus sp. G1 for enhancing WAS hydrolysis was conducted in this study with uniform design and response surface methodology. Taking the lysis rate of Escherichia coli as the response, the Plackett-Burman design was used to screen the most important variables. Experimental results showed that the maximum predicted lysis rate of E. coli was 50.9% for 4 h treatment time with concentrations of skim milk, NaCl and NH4SO4 at 10.78, 4.36 and 11.28 g/L, respectively. The optimized dosage ratio of Geobacillus sp. G1 to WAS was 35%:65% (V-G1:V-WAS). Under this condition, soluble protein was increased to 695 mg chemical oxygen demand (COD)/L, which was 5.0 times higher than that obtained in the control (140 mg COD/L). The corresponding protease activity reached 1.1 Eu/mL. Scanning electron microscopy showed that abundant cells were apparently lysed with treatment of Geobacillus sp. G1.
WOS记录号	WOS:000341182300003
公开日期	2015-03-26
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9670]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Liu, Wenzong. Optimized culture condition for enhancing lytic performance of waste activated sludge by Geobacillus sp G1[J]. WATER SCIENCE AND TECHNOLOGY,2014,70(2):200-208.
APA	Liu, Wenzong.(2014).Optimized culture condition for enhancing lytic performance of waste activated sludge by Geobacillus sp G1.WATER SCIENCE AND TECHNOLOGY,70(2),200-208.
MLA	Liu, Wenzong."Optimized culture condition for enhancing lytic performance of waste activated sludge by Geobacillus sp G1".WATER SCIENCE AND TECHNOLOGY 70.2(2014):200-208.