Microbial community structure and function of Nitrobenzene reduction biocathode in response to carbon source switchover

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Microbial community structure and function of Nitrobenzene reduction biocathode in response to carbon source switchover
	Cheng, Haoyi; Liu, Wenzong
刊名	WATER RESEARCH
	2014
卷号	54 期号:1 页码:137-148
关键词	Biocathode Nitrobenzene reduction Microbial community structure Function Carbon source switchover
ISSN号	0043-1354
中文摘要	The stress of poised cathode potential condition and carbon source switchover for functional biocathode microbial community influences is poorly understood. Using high-throughput functional gene array (GeoChip v4.2) and Illumina 16S rRNA gene MiSeq sequencing, we investigated the phylogenetic and functional microbial community of the initial inoculum and biocathode for bioelectrochemical reduction of nitrobenzene to less toxic aniline in response to carbon source switchover (from organic glucose to inorganic bicarbonate). Selective transformation of nitrobenzene to aniline maintained in the bicarbonate fed biocathode although nitrobenzene reduction rate and aniline formation rate were significantly decreased compared to those of the glucose-fed biocathode. When the electrical circuit of the glucose-fed biocathode was disconnected, both rates of nitrobenzene reduction and of aniline formation were markedly decreased, confirming the essential role of an applied electric field for the enhancement of nitrobenzene reduction. The stress of poised cathode potential condition led to clear succession of microbial communities from the initial inoculum to biocathode and the carbon source switchover obviously changed the microbial community structure of biocathode. Most of the dominant genera were capable of reducing nitroaromatics to the corresponding aromatic amines regardless of the performance mode. Heterotrophic Enterococcus was dominant in the glucose-fed biocathode while autotrophic Paracoccus and Variovorax were dominant in the bicarbonate-fed biocathode. Relatively higher intensity of diverse multi-heme cytochrome c (putatively involved in electrons transfer) and carbon fixation genes was observed in the biocarbonate-fed biocathode, likely met the requirement of the energy conservation and maintained the nitrobenzene selective reduction capability after carbon source switchover. Extracellular pilin, which are important for biofilm formation and potential conductivity, had a higher gene abundance in the glucose-fed biocathode might explain the enhancement of electro-catalysis activity for nitrobenzene reduction with glucose supply. Dominant nitroaromatics-reducing or electrochemically active bacteria and diverse functional genes related to electrons transfer and nitroaromatics reduction were associated with nitrobenzene reduction efficiency of biocathode communities in response to carbon source switchover. (C) 2014 Elsevier Ltd. All rights reserved.
WOS记录号	WOS:000334003000014
公开日期	2015-03-26
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9668]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Cheng, Haoyi,Liu, Wenzong. Microbial community structure and function of Nitrobenzene reduction biocathode in response to carbon source switchover[J]. WATER RESEARCH,2014,54(1):137-148.
APA	Cheng, Haoyi,&Liu, Wenzong.(2014).Microbial community structure and function of Nitrobenzene reduction biocathode in response to carbon source switchover.WATER RESEARCH,54(1),137-148.
MLA	Cheng, Haoyi,et al."Microbial community structure and function of Nitrobenzene reduction biocathode in response to carbon source switchover".WATER RESEARCH 54.1(2014):137-148.