Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 土壤环境科学实验室

	Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803
	Xue, Xi-Mei ; Raber, Georg ; Foster, Simon ; Chen, Song-Can ; Francesconi, Kevin A. ; Zhu, Yong-Guan
刊名	ENVIRONMENTAL CHEMISTRY
	2014
卷号	11 期号:5 页码:506-513
关键词	arsenic
ISSN号	1448-2517
中文摘要	Although methylated arsenic and arsenosugars have been verified in various freshwater organisms, lipid-soluble arsenic compounds have not been identified. Here, we report investigations with the model organism cyanobacterium Synechocystis sp. PCC 6803 wild type and arsM (arsenic(III) S-adenosylmethionine methyltransferase) mutant strain, which lacks the enzymes for arsenic methylation cultured in various concentrations of arsenate (As-V). Although Synechocystis accumulated higher arsenic concentrations at the higher exposure levels, the bioaccumulation factor decreased with increasing As-V. The accumulated arsenic in the cells was partitioned into water-soluble and lipid-soluble fractions; lipid-soluble arsenic was found in Synechocystis wild type cells (3-35% of the total depending on the level of arsenic exposure), but was not detected in Synechocystis arsM mutant strain showing that ArsM was required for arsenolipid biosynthesis. The arsenolipids present in Synechocystis sp. PCC 6803 were analysed by high performance liquid chromatography-inductively coupled plasma-mass spectrometry, high performance liquid chromatography-electrospray mass spectrometry, and high resolution tandem mass spectrometry. The two major arsenolipids were characterised as arsenosugar phospholipids based on their assigned molecular formulas C47H88O14AsP and C47H90O14AsP, and tandem mass spectrometric data demonstrated the presence of the phosphate arsenosugar and acylated glycerol groups.
WOS记录号	WOS:000342891400004
公开日期	2015-03-26
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9633]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Xue, Xi-Mei,Raber, Georg,Foster, Simon,et al. Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803[J]. ENVIRONMENTAL CHEMISTRY,2014,11(5):506-513.
APA	Xue, Xi-Mei,Raber, Georg,Foster, Simon,Chen, Song-Can,Francesconi, Kevin A.,&Zhu, Yong-Guan.(2014).Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803.ENVIRONMENTAL CHEMISTRY,11(5),506-513.
MLA	Xue, Xi-Mei,et al."Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803".ENVIRONMENTAL CHEMISTRY 11.5(2014):506-513.