Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism
	Zhao, Bailin ; Yang, Ying ; Wang, Xiaoli ; Chong, Zechen ; Yin, Ruichuan ; Song, Shu-Hui ; Zhao, Chao ; Li, Cuiping ; Huang, Hua ; Sun, Bao-Fa ; Wu, Danni ; Jin, Kang-Xuan ; Song, Maoyong ; Zhu, Ben-Zhan ; Jiang, Guibin ; Danielsen, Jannie M. Rendtlew ; Xu, Guo-Liang ; Yang, Yun-Gui ; Wang, Hailin
刊名	NUCLEIC ACIDS RESEARCH
	2014
卷号	42 期号:3 页码:1593-1605
ISSN号	0305-1048
中文摘要	DNA methylation has been proven to be a critical epigenetic mark important for various cellular processes. Here, we report that redox-active quinones, a ubiquitous class of chemicals found in natural products, cancer therapeutics and environment, stimulate the conversion of 5mC to 5hmC in vivo, and increase 5hmC in 5751 genes in cells. 5hmC increase is associated with significantly altered gene expression of 3414 genes. Interestingly, in quinone-treated cells, labile iron-sensitive protein ferritin light chain showed a significant increase at both mRNA and protein levels indicating a role of iron regulation in stimulating Tet-mediated 5mC oxidation. Consistently, the deprivation of cellular labile iron using specific chelator blocked the 5hmC increase, and a delivery of labile iron increased the 5hmC level. Moreover, both Tet1/Tet2 knockout and dimethylox-alylglycine- induced Tet inhibition diminished the 5hmC increase. These results suggest an iron-regulated Tet-dependent DNA demethylation mechanism mediated by redox-active biomolecules.
WOS记录号	WOS:000331138800021
公开日期	2015-03-24
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9443]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhao, Bailin,Yang, Ying,Wang, Xiaoli,et al. Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism[J]. NUCLEIC ACIDS RESEARCH,2014,42(3):1593-1605.
APA	Zhao, Bailin.,Yang, Ying.,Wang, Xiaoli.,Chong, Zechen.,Yin, Ruichuan.,...&Wang, Hailin.(2014).Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism.NUCLEIC ACIDS RESEARCH,42(3),1593-1605.
MLA	Zhao, Bailin,et al."Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism".NUCLEIC ACIDS RESEARCH 42.3(2014):1593-1605.