High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation
	Wang, Feng-Hua ; Qiao, Min ; Su, Jian-Qiang ; Chen, Zheng ; Zhou, Xue ; Zhu, Yong-Guan
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2014
卷号	48 期号:16 页码:9079-9085
ISSN号	0013-936X
中文摘要	Reclaimed water irrigation (RWI) in urban environments is becoming popular, due to rapid urbanization and water shortage. The continuous release of residual antibiotics and antibiotic resistance genes (ARGs) from reclaimed water could result in the dissemination of ARGs in the downstream environment. This study provides a comprehensive profile of ARGs in park soils exposed to RWI through a high-throughput quantitative PCR approach. 147 ARGs encoding for resistance to a broad-spectrum of antibiotics were detected among all park soil samples. Aminoglycoside and beta-lactam were the two most dominant types of ARGs, and antibiotic deactivation and efflux pump were the two most dominant mechanisms in these RWI samples. The total enrichment of ARGs varied from 99.3-fold to 8655.3-fold compared to respective controls. Six to 60 ARGs were statistically enriched among these RWI samples. Four transposase genes were detected in RWI samples. TnpA-04 was the most enriched transposase gene with an enrichment was up to 2501.3-fold in Urumqi RWI samples compared with control soil samples. Furthermore, significantly positive correlation was found between ARGs and transposase abundances, indicating that transposase might be involved in the propagation of ARGs. This study demonstrated that RWI resulted in the enrichment of ARGs in urban park soils.
WOS记录号	WOS:000340701800019
公开日期	2015-03-23
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9166]
专题	生态环境研究中心_城市与区域生态国家重点实验室生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Wang, Feng-Hua,Qiao, Min,Su, Jian-Qiang,et al. High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2014,48(16):9079-9085.
APA	Wang, Feng-Hua,Qiao, Min,Su, Jian-Qiang,Chen, Zheng,Zhou, Xue,&Zhu, Yong-Guan.(2014).High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation.ENVIRONMENTAL SCIENCE & TECHNOLOGY,48(16),9079-9085.
MLA	Wang, Feng-Hua,et al."High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation".ENVIRONMENTAL SCIENCE & TECHNOLOGY 48.16(2014):9079-9085.