Inhibition of G-quadruplex assembling by DNA ligation: A versatile and non-covalent labeling strategy for bioanalysis

	Inhibition of G-quadruplex assembling by DNA ligation: A versatile and non-covalent labeling strategy for bioanalysis
	Ren, Jiangtao ; Wang, Jiahai ; Wang, Jin ; Wang,Erkang
刊名	biosensors & bioelectronics
	2014
卷号	51 期号:1 页码:336-342
关键词	NICOTINAMIDE ADENINE-DINUCLEOTIDE ROLLING CIRCLE AMPLIFICATION SELECTIVE DETECTION FLUORESCENCE DETECTION MOLECULAR BEACON CHAIN-REACTION ATP ASSAY SENSITIVITY APTAMERS DNAZYME
通讯作者	wang,j
英文摘要	through tuning relative thermodynamic stabilities (i, ii and iii), dna ligation was coupled to split g-quadruplex probes and a versatile, non-covalent labelling and fluorescent strategy was constructed based on inhibition of template-directed g-quadruplex assembling by ligation reaction. the non-covalent complex between g-quadruplex and fluorescent probe was employed as signalling label and thus covalent modification of dna probes with fluorescent probes was avoided. selective detection of small biomolecules (atp and nad(+)) in the nanomolar range was realized due to the cofactor-dependent activity of dna ligases (t4 and escherichia coli dna ligase). by virtue of the simple strategy, the effect of mismatch position of single-base mismatched template dna on the ligation efficiency was validated. meanwhile, highly mismatch-influenced ligation efficiency of ligase endows the cost-effective strategy great potential for single-nucleotide polymorphism (snp) analysis. the non-covalent labeling strategy provides a versatile and cost-effective platform for monitor of dna ligation, cofactor detection, snp analysis and other ligase-based assays. (c) 2013 published by elsevier b.v.
收录类别	SCI
语种	英语
WOS记录号	WOS:000326905500051
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/51051]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Ren, Jiangtao,Wang, Jiahai,Wang, Jin,et al. Inhibition of G-quadruplex assembling by DNA ligation: A versatile and non-covalent labeling strategy for bioanalysis[J]. biosensors & bioelectronics,2014,51(1):336-342.
APA	Ren, Jiangtao,Wang, Jiahai,Wang, Jin,&Wang,Erkang.(2014).Inhibition of G-quadruplex assembling by DNA ligation: A versatile and non-covalent labeling strategy for bioanalysis.biosensors & bioelectronics,51(1),336-342.
MLA	Ren, Jiangtao,et al."Inhibition of G-quadruplex assembling by DNA ligation: A versatile and non-covalent labeling strategy for bioanalysis".biosensors & bioelectronics 51.1(2014):336-342.