Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA
	Chen J(陈佳)1,2 ; Qiu HD(邱洪灯)1,2 ; Zhang ML(张明亮)1,2; Gu TN(顾桐年)1,2; Shao SJ(邵士俊)1,2 ; Huang Y(黄勇)3; Zhao SL(赵书林)3
刊名	Biosensors and Bioelectronics
	2015
卷号	68 页码:550-555
关键词	P53 DNA Hairpin assembly DNA machine Label-free Chemiluminescence
ISSN号	0956-5663
通讯作者	邱洪灯
英文摘要	DNA plays important regulatory roles in many life activities. Here, we have developed a novel label-free, ultrasensitive and specific chemiluminescence (CL) assay protocol for DNA detection based on hairpin assembly-triggered cyclic activation of a DNA machine. The system involves two hairpin structures, H1 and H2. Firstly, a target DNA binds with and opens the hairpin structure of H1. Then, H2 hybridizes with H1 and displaces the target DNA, which is used to trigger another new hybridization cycle between H1 and H2, leading to the generation of numerous H1–H2 complexes. The generated H1–H2 complexes are further activated with the help of polymerase and nicking enzyme, continuously yielding a large amount of G-riched DNA fragments. The G-riched DNA fragment products interact with hemin to form the activated HRP-mimicking DNA zymes that can catalyze the oxidation of luminal by H₂O₂ to produce strong CL signal resulting in an amplified sensing process. Our newly proposed homogeneous assay enables the quantitative measurement of p53 DNA (as a model) with a detection limit of 0.85 fM, which is at least 5 orders of magnitude lower than that of traditional unamplified homogeneous optical approaches. Moreover, this assay exhibits high discrimination ability even against a single base mismatch. In addition, this strategy is also capable of detecting p53 DNA in complex biological samples. The proposed sensing approach might hold a great promise for further applications in biomedical research and early clinical diagnosis.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the “Hundred Talents Program” of the Chinese Academy of Science;the National Natural Science Foundation of China (Nos.21405163;21475142);the Foundation for Sci & Tech Research Project of Gansu Province (145RJYA271)
语种	英语
WOS记录号	WOS:000351248300078
公开日期	2015-03-11
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/7159]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Guangxi Normal Univ, Coll Chem & Pharm, Key Lab Chem & Mol Engn Med Resources, Minist Educ China, Guilin 541004, Peoples R China
推荐引用方式 GB/T 7714	Chen J,Qiu HD,Zhang ML,et al. Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA[J]. Biosensors and Bioelectronics,2015,68:550-555.
APA	Chen J.,Qiu HD.,Zhang ML.,Gu TN.,Shao SJ.,...&Zhao SL.(2015).Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA.Biosensors and Bioelectronics,68,550-555.
MLA	Chen J,et al."Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA".Biosensors and Bioelectronics 68(2015):550-555.