Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique
	Zhou, Jing 1,2; Wang, Wencai 3; Liu, Mengqi 4; Liu, Zhenwen 5
刊名	PHARMACOGNOSY MAGAZINE
	2014-10-01
卷号	10 期号:40 页码:385-390
关键词	Apiaceae DNA barcoding identification nrDNA ITS Peucedanumpraeruptorum L
ISSN号	0973-1296
通讯作者	Liu,ZW (reprint author),Chinese Acad Sci,Kunming Inst Bot,Lanhei Rd 132,Kunming 650201,Peoples R China.
产权排序	第三
英文摘要	Background: Peucedanum praeruptorum L., a traditional Chinese medicine known as Qian-hu, is commonly used for dispelling wind-heat and expectorant and loss of energy. However, due to similar morphological characters and high market demand, there are many substitutes and adulterants of P. praeruptorum. DNA barcoding is an approach to identify species based on sequences from a short, standardized DNA region. Objective: To authenticate P. praeruptorum from its substitutes and adulterants. Materials and Methods: The differential identification of P. praeruptorum and 13 regional substitutes and 23 adulterants was investigated by means of DNA sequence analysis of internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA), a bootstrap neighbor-joining (NJ) tree according to Kimuras 2-parameter method was also calculated. Results: The data showed that P. praeruptorum, its substitutes and adulterants could be easily distinguished at the DNA level, while almost all species were well resolved, and successfully identified on the NJ tree. Conclusion: The ITS sequence can be used for the identification of P. praeruptorum and to distinguish it from common substitutes and adulterants.
学科主题	Chemistry, Medicinal
类目[WOS]	Chemistry, Medicinal
研究领域[WOS]	Pharmacology & Pharmacy
关键词[WOS]	INTERNAL TRANSCRIBED SPACER ; NUCLEAR RIBOSOMAL DNA ; SEQUENCE-ANALYSIS ; APIACEAE ; RDNA ; IDENTIFICATION ; PHYLOGENY ; ANGELICA ; MARKERS ; REGION
收录类别	SCI
资助信息	National NSFC [31300569]; "Hundreds of Talents" program of the Chinese Academy of Sciences
语种	英语
WOS记录号	WOS:000344498300001
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/18451]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China 2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China 4.Henan Univ Tradit Chinese Med, Zhengzhou 450046, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Jing,Wang, Wencai,Liu, Mengqi,et al. Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique[J]. PHARMACOGNOSY MAGAZINE,2014,10(40):385-390.
APA	Zhou, Jing,Wang, Wencai,Liu, Mengqi,&Liu, Zhenwen.(2014).Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique.PHARMACOGNOSY MAGAZINE,10(40),385-390.
MLA	Zhou, Jing,et al."Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique".PHARMACOGNOSY MAGAZINE 10.40(2014):385-390.