Identification of apoptosis-related genes and transcription variations in response to microcystin-LR in zebrafish liver | |
Wei, Lili1,2; Hoole, Dave3; Sun, Baojian2 | |
刊名 | TOXICOLOGY AND INDUSTRIAL HEALTH |
2014-10-01 | |
卷号 | 30期号:9页码:777-784 |
关键词 | Microcystin-LR apoptosis microarray real-time PCR zebrafish |
ISSN号 | 0748-2337 |
英文摘要 | There is growing evidence that the effects of microcystin-LR (MC-LR) are closely related to apoptosis. This study utilized microarray to identify the apoptosis-related genes induced by MC-LR in zebrafish liver. The messenger RNA abundance of some apoptosis-related genes was found to be increased, including five tumor necrosis factor (TNF)-related members (apoptosis regulatory protein siva, tumor necrosis factor-alpha (tnfa) TNF (ligand) superfamily member 10 (tnfsf10), TNF-inducible protein 6 (tnfaip6) and TNF receptor associated factor 2 binding protein (traf2bp)), three p53-related genes (tumor protein p53 inducible nuclear protein 1 (tp53inp1), p53-induced protein phosphatase 1 (ppm1d) and a novel apoptosis stimulating protein of p53 (aspp2)), bcl 2 family members (proapoptosis gene bax and antiapoptosis gene mcl 1), caspases (caspase y (caspy) and a PYD and CARD domain-containing protein (pycard)) and the transforming growth factor beta (TGF-beta) induced apoptosis protein 2 (taip2). Real-time polymerase chain reaction was used to study the kinetic transcriptional changes in seven apoptosis-related genes. Elevated transcription of p53, tp53inp1, mcl 1 and taip2 could only be detected at 6 h, increased transcription of the antagonist molecules, bcl 2 and bax could be detected at most time points and the significant change of caspy could be found at 48 h and 72 h after stimulation. Taken together, the results obtained in the present study clearly demonstrate that large amount of apoptosis-related genes are involved in the regulation of MC-LR-induced apoptosis. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Public, Environmental & Occupational Health ; Toxicology |
研究领域[WOS] | Public, Environmental & Occupational Health ; Toxicology |
关键词[WOS] | CARP CTENOPHARYNGODON-IDELLA ; IN-VIVO ; CYANOBACTERIAL TOXIN ; CELL-DEATH ; OXIDATIVE STRESS ; DANIO-RERIO ; JNK ; P53 ; EXPRESSION ; FISH |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000342991800001 |
公开日期 | 2015-01-20 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/20355] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
作者单位 | 1.Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang, Jiangxi, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China 3.Keele Univ, Sch Life Sci, Keele ST5 5BG, Staffs, England |
推荐引用方式 GB/T 7714 | Wei, Lili,Hoole, Dave,Sun, Baojian. Identification of apoptosis-related genes and transcription variations in response to microcystin-LR in zebrafish liver[J]. TOXICOLOGY AND INDUSTRIAL HEALTH,2014,30(9):777-784. |
APA | Wei, Lili,Hoole, Dave,&Sun, Baojian.(2014).Identification of apoptosis-related genes and transcription variations in response to microcystin-LR in zebrafish liver.TOXICOLOGY AND INDUSTRIAL HEALTH,30(9),777-784. |
MLA | Wei, Lili,et al."Identification of apoptosis-related genes and transcription variations in response to microcystin-LR in zebrafish liver".TOXICOLOGY AND INDUSTRIAL HEALTH 30.9(2014):777-784. |
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