Effect of dietary chitosan on growth performance, haematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp (Carassius auratus gibelio)

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	Effect of dietary chitosan on growth performance, haematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp (Carassius auratus gibelio)
	Chen, Y.1,2; Zhu, X.1; Yang, Y.1; Han, D.1; Jin, J.1; Xie, S.1
刊名	AQUACULTURE NUTRITION
	2014-10-01
卷号	20 期号:5 页码:532-546
关键词	Aeromonas hydrophila Carassius aura ins gibelio chitosan immunity intestine microbiota intestine morphology
ISSN号	1353-5773
英文摘要	A 75-day experiment was conducted with juvenile gibel carp (Carassius auratus gibelio) (4.80 +/- 0.01 g) to evaluate effects of dietary chitosan on fish growth performance, haematology, intestine morphology and immune response. Six isonitrogenous (crude protein: 383 g kg(-1)) isolipid (97.5 a kg(-1)) and isocaloric (gross energy: 16.7 kJ g(-1)) diets were formulated to contain 0, 1800, 4000, 7500, 10 000, 20 000 mg kg(-1) chitosan, respectively. The results showed that the growth was depressed when the fish fed with 10 000 mg ka(-1) chitosan. Serum cholesterol, triglyceride and low-density lipoprotein decreased in 10 000 and 20 000 mg kg(-1) chitosan. On day 75, blood leucocyte phagocytic activity respiratory burst and alternative pathway of complement haemolytic activity were enhanced in 4000 mg kg(-1) chitosan. The number of goblet cell, intra-epithelial lymphocyte of mid-intestine and microvilli height of distal intestine increased at 4000 mg kg(-1) dietary chitosan. Dietary chitosan modulated intestine microbiota, depressed pathogen bacteria Aeromonas veronii-like and improved Cellulomonas hominis-like, Bacillus oceanisediminis-like and two uncultured bacterium-like species on day 75. Dietary 7500 and 10 000 mg kg(-1) chitosan enhanced the protection against Aeromonas hydrophila infection. In conclusion, oral administration of dietary 7500 mg kg(-1) chitosan for 75 days is recommended for the survival of gibel carp.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	ALTERNATIVE COMPLEMENT PATHWAY ; TURBOT SCOPHTHALMUS-MAXIMUS ; X OREOCHROMIS-AUREUS ; FRESH-WATER FISH ; CYPRINUS-CARPIO ; INNATE IMMUNITY ; MARINE ACTINOBACTERIA ; AEROMONAS-HYDROPHILA ; MANNAN OLIGOSACCHARIDES ; PARALICHTHYS-OLIVACEUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000341872600008
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20304]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Y.,Zhu, X.,Yang, Y.,et al. Effect of dietary chitosan on growth performance, haematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp (Carassius auratus gibelio)[J]. AQUACULTURE NUTRITION,2014,20(5):532-546.
APA	Chen, Y.,Zhu, X.,Yang, Y.,Han, D.,Jin, J.,&Xie, S..(2014).Effect of dietary chitosan on growth performance, haematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp (Carassius auratus gibelio).AQUACULTURE NUTRITION,20(5),532-546.
MLA	Chen, Y.,et al."Effect of dietary chitosan on growth performance, haematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp (Carassius auratus gibelio)".AQUACULTURE NUTRITION 20.5(2014):532-546.