Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides

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	Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides
	[Huang, Xiaohong ; Huang, Youhua ; Cai, Jia ; Wei, Shina ; Ouyang, Zhengliang ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013
卷号	34 期号:5 页码:1094-1102
关键词	ISG15 Epinephelus coioides GNNV Iridovirus ISGylation
ISSN号	1050-4648
通讯作者	qinqw@scsio.ac.cn
中文摘要	Interferon-stimulated gene 15 (ISG15) is an ubiquitin homolog that is significantly induced by type I interferons or viral infections. Groupers, Epinephelus spp. being maricultured in China and Southeast Asian countries, always suffer from virus infection, including iridovirus and nodavirus. To date, the roles of grouper genes, especially interferon related genes in virus infection remained largely unknown. Here, the ISG15 homolog (EcISG15) was cloned from grouper Epinephelus coioides and its immune response to Singapore grouper iridovirus (SGIV) and grouper nervous necrosis virus (GNNV) was investigated. The full-length EcISG15 cDNA was composed of 948 bp and encoded a polypeptide of 155 amino acids with 37-68% identity with the known ISG15 homologs from other fish species. Amino acid alignment analysis indicated that EcISG15 contained two ubiquitin-like (UBL) domains and an Ub-conjugation domain (LRGG). Expressional analysis showed that EcISG15 was dramatically induced by GNNV infection, poly I:C or poly dA-dT treatment, but no obvious changes were observed during SGIV infection. Immunofluorescence assay showed that EcISG15 localized mainly in the cytoplasm of grouper cells in response to poly I:C stimulation or GNNV infection, but not in mock or SGIV infected cells. Western blot analysis indicated that the ISGylation was absent in SGIV-infected cells, but significantly enhanced in GNNV-infected or poly I:C transfected cells, suggesting that EcISG15 might play different roles in SGIV and GNNV infection. Furthermore, overexpression of EcISG15 in vitro inhibited the transcription of GNNV genes significantly. Taken together, the results indicated that fish ISG15 might exert important roles against RNA virus infection. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助信息	This work was supported by grants from the National Basic Research Program of China (973) (2012CB114404, 2012CB114402), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the National High Technology Development Program of China (863) (2012AA092201), the knowledge innovative program of the Chinese Academy of Sciences (KZCX2-EW-Q213, KZCX2-YW-BR-08).
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
语种	英语
WOS记录号	WOS:000317870600010
公开日期	2015-01-22
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/11039]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	[Huang, Xiaohong,Huang, Youhua,Cai, Jia,et al. Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1094-1102.
APA	[Huang, Xiaohong,Huang, Youhua,Cai, Jia,Wei, Shina,Ouyang, Zhengliang,&Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.(2013).Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,34(5),1094-1102.
MLA	[Huang, Xiaohong,et al."Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1094-1102.