Genetic diversity of Rhododendron delavayi var. delavayi (C. B. Clarke) Ridley inferred from nuclear and chloroplast DNA: implications for the conservation of fragmented populations

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	Genetic diversity of Rhododendron delavayi var. delavayi (C. B. Clarke) Ridley inferred from nuclear and chloroplast DNA: implications for the conservation of fragmented populations
	Sharma, Anup 1,2,3; Poudel, Ram Chandra 3,4; Li, Airong1,2 ; Xu, Jianchu1,2,3 ; Guan, Kaiyun 1,2,5
刊名	PLANT SYSTEMATICS AND EVOLUTION
	2014-10-01
卷号	300 期号:8 页码:1853-1866
关键词	Rhododendron delavayi var. delavayi Genetic diversity Habitat fragmentation Yungui plateau Chloroplast and nuclear DNA Conservation
ISSN号	0378-2697
通讯作者	Guan,KY (reprint author),Chinese Acad Sci,Xinjiang Inst Ecol & Geog,Xinjiang 830011,Peoples R China. ; guanky@mail.kib.ac.cn
产权排序	第二
英文摘要	Rhododendron delavayi Franch. is an ecologically and horticulturally important tree species distributed in a global biodiversity hotspot in southwest China, an area with high level of human-induced habitat degradation and fragmentation. In an attempt to explore the level of genetic diversity and population differentiation in the remnant populations of R. delavayi var. delavayi (C. B. Clarke) Ridley, we assessed patterns of chloroplast (trnS-trnG and trnL-trnF) and nuclear (RPB2-i) DNA variation in 327 individuals representing 17 natural populations from southwest China. Analyses revealed two phylogeographic groups, with similar circumscription between the two markers. We found moderate levels of genetic differentiation (cpDNA: N (ST) = 0.125; G (ST) = 0.114 and nuclear DNA: N (ST) = 0.261; G (ST) = 0.152). Both genomes demonstrate significant correlation between genetic and geographic distances (cpDNA: r (2) = 0.248, p = 0.001 and nuclear DNA: r (2) = 0.250, p = 0.001). The genetic diversity was positively associated with an increase in longitude. Populations from the eastern region of the Yungui plateau, representing potential refugial area for R. delavayi var. delavayi, registered higher haplotype diversity and allelic richness. The mismatch distribution analysis rejected rapid population expansion. The overall population expansion time for R. delavayi var. delavayi was estimated to be 0.208-0.624 mya. The complex landscape of southwest China and the human-induced fragmentation of the natural habitat have led to significant pairwise population differentiation and moderate genetic diversity (cpDNA: 0.626 and nuclear DNA: 0.506) and haplotypic richness (cpDNA: 4.01 and nuclear DNA: 2.589) in R. delavayi var. delavayi. Based on these findings, we recommend strategies for the conservation and sustainable management of R. delavayi var. delavayi.
学科主题	Plant Sciences ; Evolutionary Biology
类目[WOS]	Plant Sciences ; Evolutionary Biology
研究领域[WOS]	Plant Sciences ; Evolutionary Biology
关键词[WOS]	BIODIVERSITY CONSERVATION ; NATURAL HYBRIDIZATION ; SEQUENCE VARIATION ; STATISTICAL-METHOD ; ADJACENT REGIONS ; SOUTHWEST CHINA ; YUNNAN ; PHYLOGEOGRAPHY ; ERICACEAE ; CPDNA
收录类别	SCI
资助信息	Main direction program of knowledge innovation of Chinese Academy of Sciences [KSCX2- YW-Z1019]; Key laboratory of biodiversity and biogeography, Kunming institute of botany, Chinese academy of sciences
语种	英语
WOS记录号	WOS:000341925200010
公开日期	2014-11-25
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/18415]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650204, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 830011, Peoples R China 5.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Xinjiang 830011, Peoples R China
推荐引用方式 GB/T 7714	Sharma, Anup,Poudel, Ram Chandra,Li, Airong,et al. Genetic diversity of Rhododendron delavayi var. delavayi (C. B. Clarke) Ridley inferred from nuclear and chloroplast DNA: implications for the conservation of fragmented populations[J]. PLANT SYSTEMATICS AND EVOLUTION,2014,300(8):1853-1866.
APA	Sharma, Anup,Poudel, Ram Chandra,Li, Airong,Xu, Jianchu,&Guan, Kaiyun.(2014).Genetic diversity of Rhododendron delavayi var. delavayi (C. B. Clarke) Ridley inferred from nuclear and chloroplast DNA: implications for the conservation of fragmented populations.PLANT SYSTEMATICS AND EVOLUTION,300(8),1853-1866.
MLA	Sharma, Anup,et al."Genetic diversity of Rhododendron delavayi var. delavayi (C. B. Clarke) Ridley inferred from nuclear and chloroplast DNA: implications for the conservation of fragmented populations".PLANT SYSTEMATICS AND EVOLUTION 300.8(2014):1853-1866.