Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus

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	Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus
	Ren, CH ; Chen, T ; Jiang, X ; Wang, YH ; Hu, CQ
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014
卷号	38 期号:1 页码:265-274
关键词	Ferritin Stichopus monotuberculatus Anti-oxidation activity Iron binding capacity Innate immune defense
ISSN号	1050-4648
通讯作者	rosemary166@sina.com ; chan1010@scsio.ac.cn ; jiangxiao4367@126.com ; yhwang@scsio.ac.cn ; hucq@scsio.ac.cn
中文摘要	Ferritin is one of the major non-harm iron storage proteins that found in most cell types of animals, plants and microorganisms. In this study, a ferritin subunit named StmFer was identified from the sea cucumber (Stichopus monotuberculatus) and characterized functionally. The full-length cDNA of StmFer is 1184 bp in size with a 5'-untranslated region (UTR) of 131 bp, a 3'-UTR of 531 bp and an open reading frame of 522 bp that encoding a protein of 173 amino acids with a deduced molecular weight of 19.95 kDa. StmFer possesses both the ferroxidase center of vertebrate ferritin heavy subunit and iron nucleation sites of vertebrate ferritin light subunit. For the gene structure, StmFer contains only three exons separated by two introns. Higher levels of mRNA expression were noticed in intestine and coelomocyte of S. monotuberculans by northern blot analysis. In in vitro experiments performed in coelomocytes, transcriptional expression of StmFer showed the strongest response to polyriboinosinic polyribocytidylic acid [Poly (I:C)] (9.08 fold up-regulation), followed by lipopolysaccharides (LPS), ferrous chloride (FeCl2) and inactivated bacteria (Vibrio alginolyticus) (7.84, 7.41 and 4.90 fold up-regulation, respectively) after 3 h post-challenge. In addition, the anti-oxidation activity and iron binding capacity of recombinant ferritin protein were demonstrated in this study. As a whole, our study suggested that the ferritin from sea cucumber may play critical roles not only in the cellular and organismic iron homeostasis, but also in the innate immune defense. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
资助信息	Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272]
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000336353700033
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10446]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Ren, CH,Chen, T,Jiang, X,et al. Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):265-274.
APA	Ren, CH,Chen, T,Jiang, X,Wang, YH,&Hu, CQ.(2014).Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus.FISH & SHELLFISH IMMUNOLOGY,38(1),265-274.
MLA	Ren, CH,et al."Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):265-274.