Morphological alterations in erythrocytes treated with silver nanoparticles biomineralized by marine sediment-derived Bacillus sp VITSSN01

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Morphological alterations in erythrocytes treated with silver nanoparticles biomineralized by marine sediment-derived Bacillus sp VITSSN01
	Revathy, T ; Saranya, R ; Jayasri, MA ; Saurav, K ; Suthindhiran, K
刊名	ANNALS OF MICROBIOLOGY
	2014
卷号	64 期号:3 页码:1291-1299
关键词	Marine microorganisms Bacillus sp VITSSN01 Silver nanoparticles Hemolytic activity Antimicrobial activity
ISSN号	1590-4261
通讯作者	ksuthindhiran@vit.ac.in
中文摘要	Biomineralization-inspired preparation of nanoparticles by marine microorganisms is in the limelight of modern nanotechnology. In recent years, the use of marine microorganisms for the synthesis of nanoparticles has been gaining importance due to the simplicity and eco-friendliness of the approach. Here we describe the synthesis of silver nanoparticles using halotolerant Bacillus sp. isolated from the southern coastal waters of India. Our selective and enriched isolation technique resulted in the isolation of a silver nitrate-resistant novel marine Bacillus sp. isolated from sediments collected at Ennore Port, Chennai, India. The strain was characterized by the polyphasic taxonomic approach, and phenotypic and phylogenetic analysis identified the strain as Bacillus sp. VITSSN01. The resistant strain was further assayed for the synthesis of silver nanoparticles and its biological activity evaluated. Nanoparticles were synthesized under optimized nutritional and cultural conditions with shaking and the production continuously monitored. The nanoparticles thus produced were then characterized by atomic force microscopy, X-ray diffraction, Fourier transform-infrared spectrophotometer and transmission electron microscopy. The mean particle size was 46 nm. Hemotological toxicity of nanoparticles is very severe form and less studied. We therefore checked the synthesized silver nanoparticles for toxicity against erythrocytes and found that the silver nanoparticles exhibited moderate hemolytic activity against human erythrocytes, with a half maximal effective concentration (EC50) value of 60 mu g/ml. Microscopic studies of the treated erythrocytes showed slight structural perturbations. The results of our study strongly suggest that marine microorganisms could be a potential source for the rapid and eco-friendly synthesis of nanoparticles.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
收录类别	SCI
原文出处	SPRINGER
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10393]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Revathy, T,Saranya, R,Jayasri, MA,et al. Morphological alterations in erythrocytes treated with silver nanoparticles biomineralized by marine sediment-derived Bacillus sp VITSSN01[J]. ANNALS OF MICROBIOLOGY,2014,64(3):1291-1299.
APA	Revathy, T,Saranya, R,Jayasri, MA,Saurav, K,&Suthindhiran, K.(2014).Morphological alterations in erythrocytes treated with silver nanoparticles biomineralized by marine sediment-derived Bacillus sp VITSSN01.ANNALS OF MICROBIOLOGY,64(3),1291-1299.
MLA	Revathy, T,et al."Morphological alterations in erythrocytes treated with silver nanoparticles biomineralized by marine sediment-derived Bacillus sp VITSSN01".ANNALS OF MICROBIOLOGY 64.3(2014):1291-1299.