Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis

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	Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis
	Wang, H ; Zhang, HD ; Mi, YL ; Ju, JH ; Chen, Q ; Zhang, HJ
刊名	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
	2014
卷号	70 页码:1402-1405
ISSN号	1744-3091
通讯作者	hjzhang@hust.edu.cn
中文摘要	beta-Carboline alkaloids (beta Cs), with tricyclic pyrido[3,4-b] indole rings, have important pharmacological and therapeutic value. In the biosynthesis of beta Cs, the Pictet-Spengler (PS) cyclization reaction is responsible for the formation of ring structures. McbB is one of a few enzymes that are known to catalyse PS cyclization. It can also catalyse decarboxylation and oxidation. Here, the expression, crystallization and preliminary data analysis of McbB are reported. The crystals diffracted to 2.10 angstrom resolution and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 66.06, b = 85.48, c = 106.19 angstrom, alpha = 90.00, beta = 106.77, gamma = 90.00 degrees. These results provide a basis for solving the crystal structure and elucidating the catalytic mechanism for McbB.
学科主题	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
收录类别	SCI
资助信息	National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326]
原文出处	WILEY-BLACKWELL
WOS记录号	WOS:000343060200020
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10377]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Wang, H,Zhang, HD,Mi, YL,et al. Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2014,70:1402-1405.
APA	Wang, H,Zhang, HD,Mi, YL,Ju, JH,Chen, Q,&Zhang, HJ.(2014).Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,70,1402-1405.
MLA	Wang, H,et al."Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70(2014):1402-1405.