Characteristic and functional analysis of a ficolin-like protein from the oyster Crassostrea hongkongensis

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	Characteristic and functional analysis of a ficolin-like protein from the oyster Crassostrea hongkongensis
	Xiang, ZM ; Qu, FF ; Wang, FX ; Li, J ; Zhang, YH ; Yu, ZN
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014
卷号	40 期号:2 页码:514-523
关键词	Crassostrea hongkongensis Ficolins ChFCN Immunity
ISSN号	1050-4648
通讯作者	carlzyu@scsio.ac.cn
中文摘要	Ficolins are a group of soluble animal proteins with multiple roles in innate immunity. These proteins recognize and bind carbohydrates in pathogens and activate the complement system, leading to opsonization, leukocyte activation, and direct pathogen killing, which have been reported in many animal species but might not be present in the shellfish lineage. In the present study, we identified the first fibrinogen-related protein from the oyster, Crassostrea hongkongensis. This novel ficolin-like protein contains a typical signal peptide and a fibrinogen-related domain (designated ChFCN) at the N and C termini, respectively, but does not contain the additional collagen-like domain of ficolins. The full-length cDNA of ChFCN is 1105 bp, encoding a putative protein of 297 amino acids with the molecular weight of 35.5 kD. ChFCN is ubiquitously expressed in selected tissues, with the highest expression level observed in the gills. The temporal expression of ChFCN following microbe infection shows that the expression of ChFCN in hemocytes increases at 3 h post-challenge. The ChFCN protein expression was also examined, and fluorescence microscopy revealed that deChFCN (truncated signal peptide) is located in the cytoplasm of HeLa cells. Full-length ChFCN was detected in the medium supernatant by western blot analysis. Recombinant ChFCN proteins with the molecular weight about 50 kD bind Saccharomyces cerevisiae, Staphylococcus haemolyticus or Escherichia coli K-12, but not those from Vibrio alginolyticus. Furthermore, the rChFCN protein could agglutinate Gram-negative bacteria E. coli K-12 and enhance the phagocytosis of C hongkongensis hemocytes in vitro. These results indicate that ChFCN might play an important role in the immunity response of oysters. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
资助信息	National Science Foundation of China [31202021, 41176150]; National Basic Research Program of China [2010CB126404, U1201215]; Program of Administration of Ocean and Fisheries of Guangdong Province, China [A201301B08]
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000343381100021
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10376]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Xiang, ZM,Qu, FF,Wang, FX,et al. Characteristic and functional analysis of a ficolin-like protein from the oyster Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):514-523.
APA	Xiang, ZM,Qu, FF,Wang, FX,Li, J,Zhang, YH,&Yu, ZN.(2014).Characteristic and functional analysis of a ficolin-like protein from the oyster Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,40(2),514-523.
MLA	Xiang, ZM,et al."Characteristic and functional analysis of a ficolin-like protein from the oyster Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):514-523.