The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas

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	The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas
	Zhang, Y ; Yu, F ; Li, J ; Tong, Y ; Zhang, YH ; Yu, ZN
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014
卷号	40 期号:2 页码:466-471
关键词	Crassostrea gigas Retinoic acid-inducible gene I Antiviral immune response PAMP challenge
ISSN号	1050-4648
通讯作者	carlzyu@scsio.ac.cn
中文摘要	Retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) is a pivotal receptor that detects numerous RNA and DNA viruses and mediates the innate induction of interferons and pro-inflammatory cytokines upon viral infection. In the present study, we cloned and characterized the first RIG-I gene in a marine mollusk, Crassostrea gigas, and designated it as CgRIG-I. The full-length CgRIG-I cDNA is 3436 bp, including 5'- and 3'-untranslated regions (UTRs) of 93 bp and 286 bp, respectively, and an open reading frame (ORF) of 3057 bp. The gene encodes a 1018 amino acid polypeptide with an estimated molecular mass of 116.5 kDa. SMART analysis showed that the CgRIG-I protein had the typical conserved domains, including the caspase activation and recruitment domains (CARDs), the RNA helicase domain and the C-terminal regulatory domain (RD). Phylogenetic analysis revealed that CgRIG-I was grouped into the clade of its vertebrate homologs. Moreover, CgRIG-I expression could be specifically increased after stimulation by poly(I:C) rather than by other PAMPs such as lipopolysaccharide (LPS), peptidoglycan (PGN), heat-killed Listeria monocytogenes (HKLM) and heat-killed Vibrio alginolyticus (HKVA). Meanwhile, six IRF, three STAT and one NF-kappa B predicted sites were identified in the CgRIG-I promoter, which was consistent with its high responsiveness to poly(I:C). In summary, this report provides the first CgRIG-I sequence of a mollusk, but its function in the antiviral immune response requires further investigation. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
资助信息	National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000343381100015
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10375]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Zhang, Y,Yu, F,Li, J,et al. The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):466-471.
APA	Zhang, Y,Yu, F,Li, J,Tong, Y,Zhang, YH,&Yu, ZN.(2014).The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,40(2),466-471.
MLA	Zhang, Y,et al."The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):466-471.