IL-8-induced L-selectin shedding regulates its binding kinetics to PSGL-1

	IL-8-induced L-selectin shedding regulates its binding kinetics to PSGL-1
	Jia XL(贾潇凌); Chen J(陈娟); Long M(龙勉)
刊名	Chinese Science Bulletin
	2009
通讯作者邮箱	mlong@imech.ac.cn
卷号	54 期号:16 页码:2786-93
ISSN号	1001-6538
通讯作者	龙勉
合作状况	其它
中文摘要	L-selectin plays a crucial role in inflammation cascade by initiating the tethering and rolling of leukocytes on endothelium wall. While many L-selectin molecules are rapidly shed from the cell surface upon activation, the remaining membrane-anchored L-selectin may still play an important role in regulating leukocyte rolling and adhesion with different binding kinetics. Here we developed an in vitro model to activate Jurkat cells via interlukin-8 (IL-8) and quantified the two-dimensional (2D) binding kinetics, using a micropipette aspiration assay, of membrane-anchored L-selectin to P-selectin glycoprotein ligand 1 (PSGL-1) ligand coupled onto human red blood cells (RBCs). The data indicated that L-selectin shedding reduced the amount of membrane-anchored L-selectin and lowered both its reverse and forward rates. These results suggested that the rolling dynamics of activated leukocytes was determined by two opposite impacts: reducing the surface presentation would enhance the rolling but lowering the kinetic rates would decrease the rolling. This finding provides a new insight into understanding how L-selectin shedding regulates leukocyte rolling and adhesion.
学科主题	生物力学
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	LYMPHOCYTE HOMING RECEPTOR ; P-SELECTIN ; GLYCOPROTEIN LIGAND-1 ; ADHESION PROTEINS ; IN-VITRO ; SURFACE ; LEUKOCYTES ; FLOW ; IDENTIFICATION ; NEUTROPHILS
收录类别	SCI
语种	英语
WOS记录号	WOS:000269212800009
公开日期	2009-12-31
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/33024]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Jia XL,Chen J,Long M. IL-8-induced L-selectin shedding regulates its binding kinetics to PSGL-1[J]. Chinese Science Bulletin,2009,54(16):2786-93.
APA	贾潇凌,陈娟,&龙勉.(2009).IL-8-induced L-selectin shedding regulates its binding kinetics to PSGL-1.Chinese Science Bulletin,54(16),2786-93.
MLA	贾潇凌,et al."IL-8-induced L-selectin shedding regulates its binding kinetics to PSGL-1".Chinese Science Bulletin 54.16(2009):2786-93.