熊果酸增强小鼠肝癌细胞疫苗免疫原性的作用

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	熊果酸增强小鼠肝癌细胞疫苗免疫原性的作用
	李艳红; 李晓波; 陆雪莹; 高剑峰; 肖向文
刊名	中国生物工程杂志
	2013
卷号	33 期号:10 页码:28-35
关键词	熊果酸佐剂小鼠H22肝癌疫苗 Ursolic acid Immunoadjuvant H22 hepatocarcinoma cells vaccine
ISSN号	1671-8135
其他题名	Immunoadjuvant Effect of Ursolic Acid for Murine H22 Hepatocarcinoma Cell Vaccine
中文摘要	目的:探讨熊果酸(UA)作为免疫佐剂对小鼠肝癌肿瘤疫苗免疫原性的增强作用.方法:UA和H22细胞共培养,制备肝癌细胞疫苗,免疫小鼠后建立肝癌模型,观察肿瘤生长曲线,记录小鼠存活情况,MTT法测定脾淋巴细胞增殖能力,ELISA法测定血清IL-2、IL-4细胞因子含量,流式细胞术测定CD4+、CD8+T细胞亚群百分率,免疫荧光法测定血清中抗肿瘤特异性抗体的产生,ELISA法测定特异性抗体的水平,Western blot检测血清特异性抗体结合能力.结果:免疫小鼠后,与模型组相比,荷瘤小鼠肿瘤的生长明显受到抑制(P＜0.05),小鼠的生命延长率明显高于模型组(P＜0.01);与模型组相比,T淋巴细胞和B淋巴细胞增殖能力显著升高(P＜0.01),CD4+/CD8+T细胞含量比例明显升高(P＜0.01),血清中细胞因子IL-2、IL-4含量明显升高(P＜0.01).免疫鼠血清可检测到较高水平特异性抗体,抗体亚型IgG2a/IgG1比值明显升高(P＜0.01);Westem blot出现4条大小约为95kDa、60kDa、50kDa和34kDa杂交条带.结论:UA作为佐剂能够提高小鼠H22肝癌细胞疫苗的免疫原性,增强小鼠抗肿瘤细胞免疫和体液免疫能力.
英文摘要	To study the immunoadjuvant effect of ursolic acid (UA) co-immunized with murine H22 hepatocarcinoma cell vaccine. The mice were injected lysate of H22 hepatocarcinoma cell and UA co-culture as vaccine three times. After one week of the last immunization the H22 cell model were set up. The tumor growth curve and survival rate of the mice were observed. The proliferations of T lymphocytes were measured by MTT and the levels of serum cytokines IL-2 and IL-4 were determined by ELISA. CD4~+, CD8~+ lymphocyte populations were determined by flow cytometry. The serum antitumor specific antibody were determined by immunofluorescence, ELISA and Western blot. It showed that after the mice were immunized with the tumor vaccines treated with UA as immunoadjuvant. Compared with model group, the tumor growth was inhibited obviously (P<0.05), the life span of vaccine group was significantly prolonged (P<0.01). The T and B lymphocyte proliferations were significantly promoted (P<0.01), the ratio of CD4~+/CD8~+ was promoted obviously (P<0.01) and the levels of serum cytokines IL-2 and IL-4 significantly higher (P<0.01).The specific binding of antiserum and antigen was found and the higher levels of serum antibody was detected. It is concluded that UA as adjuvant could remarkably improves the immunogenicity of H22 tumor vaccines and the mice cellular immunity and humoral immunity could enhance on antitumor.
收录类别	CSCD
资助信息	新疆维吾尔自治区自然科学基金(2011211B51);中国科学院资源环境科学与技术局重要创新方向“西部行动”项目(KZCX-XB2-17)资助项目
CSCD记录号	CSCD:4975052
公开日期	2014-11-11
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/3666]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	石河子大学动物科技学院；中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室
推荐引用方式 GB/T 7714	李艳红,李晓波,陆雪莹,等. 熊果酸增强小鼠肝癌细胞疫苗免疫原性的作用[J]. 中国生物工程杂志,2013,33(10):28-35.
APA	李艳红,李晓波,陆雪莹,高剑峰,&肖向文.(2013).熊果酸增强小鼠肝癌细胞疫苗免疫原性的作用.中国生物工程杂志,33(10),28-35.
MLA	李艳红,et al."熊果酸增强小鼠肝癌细胞疫苗免疫原性的作用".中国生物工程杂志 33.10(2013):28-35.