Roles of human sulfotransferases in genotoxicity of carcinogens using genetically engineered umu test strains

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	Roles of human sulfotransferases in genotoxicity of carcinogens using genetically engineered umu test strains
	Zhang, Yu
刊名	Environmental Sciences & Ecology; Genetics & Heredity; Toxicology
	2012
卷号	53 期号:2 页码:152-164
关键词	human sulfotransferases carcinogens genotoxicity umu test
ISSN号	0893-6692
中文摘要	Human sulfotransferase (SULT) 1A1, 1A2, and 1A3 cDNA genes were subcloned separately into the pTrc99AKM vector. The generated plasmids were introduced into the Salmonella typhimurium O-acetyltransferase-deficient strain NM6000 (TA1538/1,8-DNP/pSK1002), resulting in the new strains NM7001, NM7002, and NM7003. We compared the sensitivities of these three strains with the parental strain NM7000 against 51 chemicals including aromatic amines, nitroarenes, alkenylbenzenes, estrogens-like chemicals, and other compounds with and without S9 mix by making use of the umu test system that is based on the bacterial SOS induction. 2-Amino-6-methyl-dipyrido[1,2-a:3',2'-d]imidazole, 3-methoxy-4-aminoazobenzene, 3-nitrobenzanthrone, 5-nitroacenaphthene, and 3,9-dinitrofluoranthene caused high genotoxicity in the NM7001 strain. The genotoxic effects of 2-aminofluorene, 2-acetylaminofluorene, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, 2-nitrofluorene, 1-nitropyrene, and 2-nitropropane were stronger in the NM7002 strain compared with the NM7001 and NM7003 strains. Among the tested benzylic and allylic compounds, 1-hydroxymethylpyrene was detected in the NM7001 strain with the highest sensitivity. Estragole and 1'-hydroxysafrole exhibited strong genotoxicity in the NM7003 strain. The estrogen-like chemicals such as bisphenol A, genistein, p,n-nonylphenol, and 4-hydroxytamoxifen were not detected as genotoxins in any strain used. Collectively, the present results suggest that the generated test strains are valuable tools in order to elucidate the role of SULT enzymes in the bioactivation of chemicals to environmental carcinogens. Environ. Mol. Mutagen. 2012. (c) 2011 Wiley Periodicals, Inc.
WOS记录号	WOS:000299831600008
公开日期	2014-11-28
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/8171]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Zhang, Yu. Roles of human sulfotransferases in genotoxicity of carcinogens using genetically engineered umu test strains[J]. Environmental Sciences & Ecology; Genetics & Heredity; Toxicology,2012,53(2):152-164.
APA	Zhang, Yu.(2012).Roles of human sulfotransferases in genotoxicity of carcinogens using genetically engineered umu test strains.Environmental Sciences & Ecology; Genetics & Heredity; Toxicology,53(2),152-164.
MLA	Zhang, Yu."Roles of human sulfotransferases in genotoxicity of carcinogens using genetically engineered umu test strains".Environmental Sciences & Ecology; Genetics & Heredity; Toxicology 53.2(2012):152-164.