Sensitive immunoassay detection of multiple environmental chemicals on protein microarrays using DNA/dye conjugate as a fluorescent label

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Sensitive immunoassay detection of multiple environmental chemicals on protein microarrays using DNA/dye conjugate as a fluorescent label
	Fan, Ziyan ; Keum, Young Soo ; Li, Qing X. ; Shelver, Weilin L. ; Guo, Liang-Hong
刊名	JOURNAL OF ENVIRONMENTAL MONITORING
	2012
卷号	14 期号:5 页码:1345－1352
ISSN号	1464-0325
中文摘要	Indirect competitive immunoassays were developed on protein microarrays for the sensitive and simultaneous detection of multiple environmental chemicals in one sample. In this assay, a DNA/SYTOX Orange conjugate was employed as an antibody label to increase the fluorescence signal and sensitivity of the immunoassays. Epoxy-modified glass slides were selected as the substrate for the production of 4 x 4 coating antigen microarrays. With this signal-enhancing system, competition curves for 17 beta-estradiol (E2), benzo[a]pyrene (BaP) and 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) were obtained individually on the protein microarray. The IC50 and calculated limit of detection (LOD) are 0.32 mu g L-1 and 0.022 mu g L-1 for E2, 37.2 mu g L-1 and 24.5 mu g L-1 for BaP, and 31.6 mu g L-1 and 2.8 mu g L-1 for BDE-47, respectively. LOD of E2 is 14-fold lower than the value reported in a previous study using Cy3 labeled antibody (Du et al., Clin. Chem, 2005, 51, 368-375). The results of the microarray immunoassay were within 15% of chromatographic analysis for all three pollutants in spiked river water samples, thus verifying the immunoassay. Simultaneous detection of E2, BaP and BDE-47 in one sample was demonstrated. There was no cross-reaction in the immunoassay between these three environmental chemicals. These results suggest that microarray-based immunoassays with DNA/dye conjugate labels are useful tools for the rapid, sensitive, and high throughput screening of multiple environmental contaminants.
WOS记录号	WOS:000303501800009
公开日期	2014-11-04
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/7755]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Fan, Ziyan,Keum, Young Soo,Li, Qing X.,et al. Sensitive immunoassay detection of multiple environmental chemicals on protein microarrays using DNA/dye conjugate as a fluorescent label[J]. JOURNAL OF ENVIRONMENTAL MONITORING,2012,14(5):1345－1352.
APA	Fan, Ziyan,Keum, Young Soo,Li, Qing X.,Shelver, Weilin L.,&Guo, Liang-Hong.(2012).Sensitive immunoassay detection of multiple environmental chemicals on protein microarrays using DNA/dye conjugate as a fluorescent label.JOURNAL OF ENVIRONMENTAL MONITORING,14(5),1345－1352.
MLA	Fan, Ziyan,et al."Sensitive immunoassay detection of multiple environmental chemicals on protein microarrays using DNA/dye conjugate as a fluorescent label".JOURNAL OF ENVIRONMENTAL MONITORING 14.5(2012):1345－1352.