Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

	Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production
	Pu, X ; Qu, XX ; Chen, F ; Bao, JK ; Zhang, GL ; Luo, YG
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2013
卷号	97 期号:21 页码:9365-9375
关键词	Camptothecin Endophytic fungi Trichoderma atroviride LY357 Fermentation Elicitor Adsorbent resin
ISSN号	0175-7598
通讯作者	Zhang, GL ; Luo, YG
产权排序	1
中文摘要	Camptothecin (CPT), the third largest anticancer drug, is produced mainly by Camptotheca acuminata and Nothapodytes foetida. CPT itself is the starting material for clinical CPT-type drugs, but the plant-derived CPT cannot support the heavy demand from the global market. Research efforts have been made to identify novel sources for CPT. In this study, three CPT-producing endophytic fungi, Aspergillus sp. LY341, Aspergillus sp. LY355, and Trichoderma atroviride LY357, were isolated and identified from C. acuminata. Most CPT produced by these fungi was found in the fermentation broth, and their corresponding CPT yields were 7.93, 42.92, and 197.82 mu g l(-1), respectively. The CPT-producing capability of LY341 and LY355 was completely lost after repeat subculturing. A substantial decrease of CPT production was also observed in the second generation of LY357. However, a stable and sustainable production of CPT was found from the second generation through the eighth generation of LY357. The fermentation medium, time, pH, temperature, and agitation rate were optimized for CPT production. Methyl jasmonate and XAD16 were proven to be an optimum elicitor and adsorbent resin, respectively, in view of that CPT yield was increased 3.4- and 11-fold through their use. A 50- to 75-fold increase of CPT yield was obtained when the optimized fermentation conditions, elicitor, and adsorbent resin were combined and applied to the culture of the seventh and eighth generations of LY357, and the highest CPT yield was 142.15 mu g l(-1). The CPT-producing T. atroviride LY357 paves a potential to uncover the mysteries of CPT biosynthesis.
学科主题	Biotechnology & Applied Microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000325617900007
公开日期	2014-11-21
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/24207]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Pu, X,Qu, XX,Chen, F,et al. Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(21):9365-9375.
APA	Pu, X,Qu, XX,Chen, F,Bao, JK,Zhang, GL,&Luo, YG.(2013).Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(21),9365-9375.
MLA	Pu, X,et al."Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.21(2013):9365-9375.