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	Konjac glucomannan microspheres for low-cost desalting of protein solution
	Xiong, Zhidong1,2; Zhou, Weiqing1; Sun, Lijing1; Li, Xiunan1; Zhao, Dawei1,2; Chen, Yi1,2; Li, Juan1; Ma, Guanghui1; Su, Zhiguo1
刊名	CARBOHYDRATE POLYMERS
	2014-10-13
卷号	111期号:OCT页码:56-62
关键词	Konjac glucomannan Gel filtration chromatography Desalting
ISSN号	0144-8617
其他题名	Carbohydr. Polym.
中文摘要	In this study, low-cost konjac glucomannan (KGM) microspheres used for desalting were developed by an inverse dispersion method. High concentration of KGM was pretreated to reduce viscosity by acid hydrolysis method under the condition of high temperature and pressure. The selectivity of the obtained cross-linked KGM gels with different degree of crosslinking was studied by constructing calibration curves (K-av) of standard molecular weight substances. The stability of the gels was investigated, which showed that the KGM microspheres are tolerant to a wide range of pH and stable in all commonly used aqueous buffers, and insensitive to autoclaving as well. Furthermore, protein-desalting performances of GM-1250, a cross-linked KGM microsphere, were evaluated with two proteins, bovine serum albumin and filamentous hemagglutinin, which turned out that GM-1250 is comparable to the widely used commercial product - Sephadex G25 Fine. From economic considerations, KGM gel is a prospective alternative for dextran gels in protein desalting process. (C) 2014 Elsevier Ltd. All rights reserved.
英文摘要	In this study, low-cost konjac glucomannan (KGM) microspheres used for desalting were developed by an inverse dispersion method. High concentration of KGM was pretreated to reduce viscosity by acid hydrolysis method under the condition of high temperature and pressure. The selectivity of the obtained cross-linked KGM gels with different degree of crosslinking was studied by constructing calibration curves (K-av) of standard molecular weight substances. The stability of the gels was investigated, which showed that the KGM microspheres are tolerant to a wide range of pH and stable in all commonly used aqueous buffers, and insensitive to autoclaving as well. Furthermore, protein-desalting performances of GM-1250, a cross-linked KGM microsphere, were evaluated with two proteins, bovine serum albumin and filamentous hemagglutinin, which turned out that GM-1250 is comparable to the widely used commercial product - Sephadex G25 Fine. From economic considerations, KGM gel is a prospective alternative for dextran gels in protein desalting process. (C) 2014 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Chemistry ; Polymer Science
关键词[WOS]	ION-EXCHANGE CHROMATOGRAPHY ; GRAFTED AGAROSE MEDIA ; DRUG-DELIVERY ; ADSORPTION ; HYDROGELS ; DIALYSIS ; MANNAN
收录类别	SCI
原文出处	://WOS:000340302100008
语种	英语
WOS记录号	WOS:000340302100008
公开日期	2014-09-30
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/11570]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiong, Zhidong,Zhou, Weiqing,Sun, Lijing,et al. Konjac glucomannan microspheres for low-cost desalting of protein solution[J]. CARBOHYDRATE POLYMERS,2014,111(OCT):56-62.
APA	Xiong, Zhidong.,Zhou, Weiqing.,Sun, Lijing.,Li, Xiunan.,Zhao, Dawei.,...&Su, Zhiguo.(2014).Konjac glucomannan microspheres for low-cost desalting of protein solution.CARBOHYDRATE POLYMERS,111(OCT),56-62.
MLA	Xiong, Zhidong,et al."Konjac glucomannan microspheres for low-cost desalting of protein solution".CARBOHYDRATE POLYMERS 111.OCT(2014):56-62.

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