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	Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix
	Qu, Jian-Bo1; Huan, Guan-Sheng1; Chen, Yan-Li1; Zhou, Wei-Qing2; Liu, Jian-Guo1; Huang, Fang1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2014-08-13
卷号	6期号:15页码:12752-12760
关键词	gigaporous coating polystyrene particles cross-linked poly(vinyl alcohol) hydrogel protein adsorption
ISSN号	1944-8244
其他题名	ACS Appl. Mater. Interfaces
中文摘要	Gigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography.
英文摘要	Gigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]	Science & Technology - Other Topics ; Materials Science
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; PERFUSION CHROMATOGRAPHY ; AFFINITY-CHROMATOGRAPHY ; STATIONARY-PHASE ; ADSORPTION ; SEPARATION ; PARTICLES ; HYDROPHILIZATION ; SUPPORTS ; DEXTRAN
收录类别	SCI
原文出处	://WOS:000340446300112
语种	英语
WOS记录号	WOS:000340446300112
公开日期	2014-09-30
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/11508]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Qu, Jian-Bo,Huan, Guan-Sheng,Chen, Yan-Li,et al. Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(15):12752-12760.
APA	Qu, Jian-Bo,Huan, Guan-Sheng,Chen, Yan-Li,Zhou, Wei-Qing,Liu, Jian-Guo,&Huang, Fang.(2014).Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix.ACS APPLIED MATERIALS & INTERFACES,6(15),12752-12760.
MLA	Qu, Jian-Bo,et al."Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix".ACS APPLIED MATERIALS & INTERFACES 6.15(2014):12752-12760.

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