Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides

	Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides
	Huang, Guang 1,2,3; Sun, Zhen 2,3; Qin, Hongqiang 2; Zhao, Liang 2; Xiong, Zhichao 2,4; Peng, Xiaojun 1; Ou, Junjie 2; Zou, Hanfa 2
刊名	analyst
	2014
卷号	139 期号:9 页码:2199-2206
ISSN号	0003-2654
通讯作者	欧俊杰 ; 邹汉法
产权排序	待补充
合作状况	英
英文摘要	hydrazide chemistry is a powerful technique in glycopeptides enrichment. however, the low density of the monolayer hydrazine groups on the conventional hydrazine-functionalized magnetic nanoparticles limits the efficiency of glycopeptides enrichment. herein, a novel magnetic nanoparticle grafted with poly(glycidyl methacrylate) (gma) brushes was fabricated via reversible addition-fragmentation chain transfer (raft) polymerization, and a large amount of hydrazine groups were further introduced to the gma brushes by ring-opening the epoxy groups with hydrazine hydrate. the resulting magnetic nanoparticles (denoted as fe3o4@sio2@gma-nhnh2) demonstrated the high specificity of capturing glycopeptides from a tryptic digest of the sample comprising a standard non-glycosylated protein bovine serum albumin (bsa) and four standard glycoproteins with a weight ratio of 50 : 1, and the detection limit was as low as 130 fmol. in the analysis of a real complex biological sample, the tryptic digest of hepatocellular carcinoma, 179 glycosites were identified by the fe3o4@sio2@gma-nhnh2 nanoparticles, surpassing that of 68 glycosites by fe3o4@sio2-single-nhnh2 (with monolayer hydrazine groups on the surface). it can be expected that the magnetic nanoparticles modified with hydrazine functionalized polymer brushes via raft technique will improve the specificity and the binding capacity of glycopeptides from complex samples, and show great potential in the analysis of protein glycosylation in biological samples.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	fragmentation chain transfer ; n-glycosylated proteins ; mass-spectrometry ; selective enrichment ; radical polymerization ; silica nanoparticles ; linked glycopeptides ; biomarker discovery ; mesoporous silica ; facile synthesis
收录类别	SCI
资助信息	2，1
原文出处	2206
语种	英语
WOS记录号	WOS:000334176400024
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119763]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 20023, Peoples R China
推荐引用方式 GB/T 7714	Huang, Guang,Sun, Zhen,Qin, Hongqiang,et al. Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides[J]. analyst,2014,139(9):2199-2206.
APA	Huang, Guang.,Sun, Zhen.,Qin, Hongqiang.,Zhao, Liang.,Xiong, Zhichao.,...&Zou, Hanfa.(2014).Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides.analyst,139(9),2199-2206.
MLA	Huang, Guang,et al."Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides".analyst 139.9(2014):2199-2206.