Enrichment of cancer stem cell-like cells by culture in alginate gel beads

	Enrichment of cancer stem cell-like cells by culture in alginate gel beads
	Xu, Xiao-xi 1,2; Liu, Chang 1,2; Liu, Yang 1; Yang, Li 3; Li, Nan 1,2; Guo, Xin 1; Sun, Guang-wei 1; Ma, Xiao-jun 1
刊名	journal of biotechnology
	2014-05-10
卷号	177 页码:1-12
关键词	Alginate gel bead Cancer stem cell Enrichment Three-dimensional culture Hypoxia-inducible factor
通讯作者	孙广炜 ; 马小军
产权排序	待补充
合作状况	英
英文摘要	cancer stem cells (cscs) are most likely the reason of cancer reoccurrence and metastasis. for further elucidation of the mechanism underlying the characteristics of cscs, it is necessary to develop efficient culture systems to culture and expand cscs. in this study, a three-dimensional (3d) culture system based on alginate gel (alg) beads was reported to enrich cscs. two cell lines derived from different histologic origins were encapsulated in alg beads respectively and the expansion of cscs was investigated. compared with two-dimensional (2d) culture, the proportion of cells with csc-like phenotypes was significantly increased in alg beads. expression levels of csc-related genes were greater in alg beads than in 2d culture. the increase of csc proportion after being cultured within alg beads was further confirmed by enhanced tumorigenicity in vivo. moreover, increased metastasis ability and higher anti-cancer drug resistance were also observed in 3d-cultured cells. furthermore, we found that it was hypoxia, through the upregulation of hypoxia-inducible factors (hifs) that occurred in alg beads to induce the increasing of csc proportion. therefore, alg bead was an efficient culture system for csc enrichment, which might provide a useful platform for csc research and promote the development of new anti-cancer therapies targeting cscs. (c) 2014 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	hepatocellular-carcinoma cells ; solid tumors ; matrix metalloproteinases ; stem/progenitor cells ; 3-dimensional culture ; composite hydrogels ; initiating cells ; drug-resistance ; hyaluronic-acid ; self-renewal
收录类别	SCI
资助信息	1，1
原文出处	12
语种	英语
WOS记录号	WOS:000333811200001
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119749]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiao-xi,Liu, Chang,Liu, Yang,et al. Enrichment of cancer stem cell-like cells by culture in alginate gel beads[J]. journal of biotechnology,2014,177:1-12.
APA	Xu, Xiao-xi.,Liu, Chang.,Liu, Yang.,Yang, Li.,Li, Nan.,...&Ma, Xiao-jun.(2014).Enrichment of cancer stem cell-like cells by culture in alginate gel beads.journal of biotechnology,177,1-12.
MLA	Xu, Xiao-xi,et al."Enrichment of cancer stem cell-like cells by culture in alginate gel beads".journal of biotechnology 177(2014):1-12.